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The Repair of Uracil-Containing DNA

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Mechanisms of DNA Damage and Repair

Part of the book series: Basic Life Sciences ((BLSC,volume 189))

Abstract

For what purpose does DNA contain thymine rather than uracil? This question has been approached by studying the consequences of the misincorporation of large amounts of uracil into DNA in place of thymine. Such studies have been facilitated by the isolation of special bacterial mutants. Let us first consider how uracil residues can appear in DNA.

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References

  1. T. Lindahl, and B. Nyberg, Heat-induced deamination of cytosine residues in deoxyribonucleic acid, Biochemistry 13: 3405–3410 (1974).

    Article  CAS  Google Scholar 

  2. H. Hayatsu, Bisulfite modification of nucleic acids and their constituents, Prog. Nucl. Acid. Res. 16: 75–124 (1976).

    Article  CAS  Google Scholar 

  3. J. Shlomai and A. Romberg, Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA, J. Bacteriol. 253: 3305–3312 (1978).

    CAS  Google Scholar 

  4. B.-K. Tye, P.O. Nyman, I.R. Lehman, S. Hochhauser, and B. Weiss, Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA, Proc. Natl. Acad. Sci. USA 74: 154–157 (1977).

    Article  CAS  Google Scholar 

  5. G.A. O’Donovan and J. Neuhard, Pyrimidine metabolism in microorganisms, Bacteriol. Rev. 34: 278–243 (1970).

    Google Scholar 

  6. S.J. Hochhauser and B. Weiss, Escherichia coli mutants deficient in deoxyuridine triphosphatase, J. Bacteriol. 134: 157–166 (1978).

    CAS  Google Scholar 

  7. T. Lindahl, DNA repair enzymes, Ann. Rev. Biochem. 51: 61–87 (1982).

    Article  CAS  Google Scholar 

  8. A.F. Taylor and B. Weiss, Role of exonuclease III in the base-excision repair of uracil-containing DNA, J. Bacteriol. 151: 351–357 (1982).

    CAS  Google Scholar 

  9. C Goffin and W.G. Verly, T4 DNA ligase can seal a nick in doublestranded DNA limited by a 5′-phosphorylated base-free deoxyribose residue, Nucleic Acids Res. 11: 8103–8109 (1983).

    Article  CAS  Google Scholar 

  10. B.-K. Tye and I.R. Lehman, Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase, J. Mol. Biol. 117: 293–306 (1977).

    Article  CAS  Google Scholar 

  11. H.R. Warner, B.F. Demple, W.A. Deutsch, CM. Kane, and S. Linn, Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA, Proc. Natl. Acad. Sci. USA 77: 4602–4606 (1980).

    Article  CAS  Google Scholar 

  12. R.P. Cunningham and B. Weiss, Endonuclease III (nth) mutants of Escherichia coli. Proc. Natl. Acad. Sci. USA 82: 474–478 (1985).

    Article  CAS  Google Scholar 

  13. G.C. Walker, Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli, Microbiol. Rev. 48: 60–93 (1984).

    CAS  Google Scholar 

  14. B.K. Duncan and B. Weiss, Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli, J. Bacteriol. 151: 750–755 (1982).

    CAS  Google Scholar 

  15. B.K. Duncan, DNA glycosylases, p. 565-586, in: “The Enzymes,” P.D. Boyer, ed., Academic Press, New York, (1981).

    Google Scholar 

  16. H.R. Warner, B.K. Duncan, C. Garrett, and J. Neuhard, Synthesis and metabolism of uracil-containing deoxyribonucleic acid in Escherichia coli, J. Bacteriol. 145: 687–695. (1981).

    CAS  Google Scholar 

  17. E.F. Fisher and M.H. Caruthers, Studies on gene control regions XII. The functional significance of a Lac operator constitutive mutation, Nucleic Acids Res. 7: 401–416 (1979).

    Article  CAS  Google Scholar 

  18. L.G. Lundberg, H.-O. Thoresson, O.H. Karlstrom, and P.O. Nyman, Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12, The EMBO Journal, 2: 967–971 (1983).

    CAS  Google Scholar 

  19. L.G. Lundberg, O.H. Karlstrom, P.O. Nyman, Isolation and characterization of the dut gene of Escherichia coli. II. Restriction enzyme mapping and analysis of polypeptide products, Gene 22: 127–131 (1983).

    Article  CAS  Google Scholar 

  20. E.D. Spitzer and B. Weiss, The dna-707 mutation of Escherichia coli, Fed. Proc. 41: 1194 (1982).

    Google Scholar 

  21. B. Weiss, Exodeoxyribonucleases of Escherichia coli, p. 203-231, in: “The Enzymes,” vol. 14, P.D. Boyer, ed., Academic Press, New York (1981).

    Google Scholar 

  22. M. Goulian, B. Bleile, and B.Y. Tseng, Methotrexate-induced misincorporation of uracil into DNA, Proc. Natl. Acad. Sci. USA 77: 1956–1960 (1980).

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Bernard Weiss & Hiyam H. El-Hajj

Authors
  1. Bernard Weiss
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  2. Hiyam H. El-Hajj
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Editor information

Editors and Affiliations

  1. National Bureau of Standards, Gaithersburg, Maryland, USA

    Michael G. Simic

  2. Chemistry Department, American University, Washington, D.C., USA

    Michael G. Simic

  3. Johns Hopkins University, Baltimore, Maryland, USA

    Lawrence Grossman

  4. New York University Medical Center, New York, New York, USA

    Arthur C. Upton

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© 1986 Plenum Press, New York

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Weiss, B., El-Hajj, H.H. (1986). The Repair of Uracil-Containing DNA. In: Simic, M.G., Grossman, L., Upton, A.C., Bergtold, D.S. (eds) Mechanisms of DNA Damage and Repair. Basic Life Sciences, vol 189. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9462-8_38

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  • DOI: https://doi.org/10.1007/978-1-4615-9462-8_38

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9464-2

  • Online ISBN: 978-1-4615-9462-8

  • eBook Packages: Springer Book Archive

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