Abstract
We have investigated how the information contained within the large RNA genome of IBV is expressed. We began by examining the structure of IBV-specified RNAs. Infected chicken embryo kidney (CEK) cells contain at least 6 IBV RNA species. These consist of the viral genome, RNA F, with an estimated complexity of 23 kb1, and subgenomic RNAs A, B, C, D, and E, which range in size from 2.4 kb to 7.9 kb2. We have recently identified an additional IBV-specified RNA species, RNA M, by Northern blot analysis using a hybridization probe containing cloned IBV cDNA sequences (see below). RNA M is intermediate in size between RNAs B and C (Table 1). Structural analysis of RNAs A, B, C, D, E, and F by ribonuclease T1 fingerprinting revealed that they comprise a 3’ coterminal nested set3. Synthesis of 3’ coterminal RNAs is now known to be a characteristic feature of Coronavirus multiplication4,5.
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© 1984 Plenum Press, New York
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Stern, D.F., Sefton, B. (1984). Organization of the IBV Genome. In: Rottier, P.J.M., van der Zeijst, B.A.M., Spaan, W.J.M., Horzinek, M.C. (eds) Molecular Biology and Pathogenesis of Coronaviruses. Advances in Experimental Medicine and Biology, vol 173. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9373-7_2
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