Abstract
It is generally accepted that a non-invasive method for measuring oxygen pressure in the heart would be a valuable aid to investigators interested in heart and circulatory physiology. We have developed an optical method for imaging the oxygen pressure in the surface layer of tissue, 0.5 to 1 mm in depth, which is based on the oxygen dependent quenching of phosphoresence (Rumsey et al., 1988; 1990; 1991; Wilson et al., 1988; 1991; 1992). From these measurements, two dimensional maps are constructed, thereby providing an accurate analysis of oxygen pressure in tissue in response to various stimuli and experimental conditions. The motion of the heart induced largely by the systolic phase of the cardiac cycle limits the time available for image acquisistion. This necessitates the synchronization of the operation of the flash lamp and imaging of the phosphorescence with the cardiac cycle. In this study we provide preliminary data that show it is possible to obtain maps of oxygen pressure in the epicardium of the heart in vivo.
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References
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© 1994 Springer Science+Business Media New York
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Rumsey, W.L., Pawlowski, M., Lejavardi, N., Wilson, D.F. (1994). Measurement of Oxygen Pressure in the Heart in Vivo Using Phosphorescence Quenching. In: Hogan, M.C., Mathieu-Costello, O., Poole, D.C., Wagner, P.D. (eds) Oxygen Transport to Tissue XVI. Advances in Experimental Medicine and Biology, vol 361. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1875-4_12
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DOI: https://doi.org/10.1007/978-1-4615-1875-4_12
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