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Taurine Chloramine Administered In Vivo Increases NRF2-Regulated Antioxidant Enzyme Expression in Murine Peritoneal Macrophages

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Taurine 8

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 775))

Abstract

Taurine chloramine (TauCl) is produced from taurine by the ­myeloperoxidase-halide system in activated neutrophils via a stoichiometric reaction between taurine and HOCl. TauCl has been shown to provide cytoprotection against inflammatory tissue injury by inhibiting the overproduction of inflammatory mediators and also by increasing the expression of antioxidant enzymes that are regulated by nuclear factor E2-related factor 2 in murine macrophages. In this study, primary murine macrophages were prepared after either by injection of 3% thioglycolate into mouse peritoneal cavity or by differentiation of the isolated bone marrow cells. TauCl increased HO-1, Prx-1, and Trx-1 expression in murine primary ­macrophages. Also, when TauCl was injected in combination with 3% thioglycolate, HO-1 expression in the peritoneal macrophages was increased. Our results suggest that TauCl plays a protective role against cytotoxicity of oxidative stress in macrophages by increasing the expression of antioxidant enzymes in vivo.

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Abbreviations

ARE:

Antioxidant response element

BMDM:

Bone marrow-derived macrophages

GPx:

Glutathione peroxidase

HO:

Heme oxygenase

Keap:

Kelch-like ECH-associated protein

LPS:

Lipopolysaccharide

M-CSF:

Macrophage colony stimulating factor

MMP:

Metalloproteinase

MPO:

Myeloperoxidase

Nrf2:

Nuclear factor E2-related factor

Prx:

Peroxiredoxin

TauCl:

Taurine chloramine

TNF-α:

Tumor necrosis factor-α

Trx:

Thioredoxin

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Acknowledgments

We thank Dr. Young-Nam Cha for discussions throughout the study and critical comments on the manuscript, and Mi Ran Cho for the technical support. This work was supported by the NRF of Korea grant funded by the Korea government MEST (2012R1A1A3007097) and Inha University research grant.

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Authors and Affiliations

  1. Laboratory for Leukocyte Signaling Research, Department of Pharmacology and Toxicology, Inha Research Institute for Medical Science, Inha University School of Medicine, Incheon, Korea

    In Soon Kang & Chaekyun Kim

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  1. In Soon Kang
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  2. Chaekyun Kim
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Correspondence to Chaekyun Kim .

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Editors and Affiliations

  1. City University of New York, Center for Developmental Neuroscience, College of Staten Island, 2800 Victory Blvd, Staten Island, 10314, New York, USA

    Abdeslem El Idrissi

  2. City University of New York, Department of Biology, College of Staten Island, Victory Blvd 2800, Staten Island, 10314, New York, USA

    William J. L'Amoreaux

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Kang, I.S., Kim, C. (2013). Taurine Chloramine Administered In Vivo Increases NRF2-Regulated Antioxidant Enzyme Expression in Murine Peritoneal Macrophages. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 775. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6130-2_22

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