Abstract
The density of Na/Ca exchangers (NCX1) in the cardiac sarcolemma, like all plasma membrane proteins, will be influenced by (and ultimately determined by) the function of membrane insertion and retrieval processes (i.e., exo- and endocytic mechanisms). Progress in understanding these processes in cardiac muscle faces many biological and methodological complexities and hurdles. As described here, we are attempting to overcome these hurdles to study more adequately the assembly and disassembly of the cardiac sarcolemma, in general, and the control of NCX1 by membrane trafficking processes in particular. First, we have developed improved noninvasive methods to monitor the cellular capacitance of cardiac tissue (NIC) over periods of hours. Thus, we can study long-term changes of total membrane area. Second, we have developed mice that express fusion proteins of NCX1 with the pHluorin green protein. Thus, we can determine the membrane disposition of NCX1, and changes thereof, on-line in intact cardiac muscle.
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References
J.M. Best, J.D. Foell, C.R. Buss, B.P. Delisle, R.C. Balijepalli, C.T. January, T.J. Kamp, The small GTPase Rab11b regulates degradation of surface membrane L-type Cav1.2 channels. Am. J. Physiol. Cell Physiol. 300, C1023–C1033 (2011)
J. Chen, J. Sroubek, Y. Krishnan, Y. Li, J. Bian, T.V. McDonald, PKA phosphorylation of HERG protein regulates the rate of channel synthesis. Am. J. Physiol. Heart Circ. Physiol. 296, H1244–H1254 (2009)
F.S. Cusdin, J.J. Clare, A.P. Jackson, Trafficking and cellular distribution of voltage-gated sodium channels. Traffic 9, 17–26 (2008)
G.J. Doherty, H.T. McMahon, Mechanisms of endocytosis. Annu. Rev. Biochem. 78, 857–902 (2009)
J.G. Donaldson, N. Porat-Shliom, L.A. Cohen, Clathrin-independent endocytosis: a unique platform for cell signaling and PM remodeling. Cell. Signal. 21, 1–6 (2009)
D.J. Fazakerley, S.P. Lawrence, V.A. Lizunov, S.W. Cushman, G.D. Holman, A common trafficking route for GLUT4 in cardiomyocytes in response to insulin, contraction and energy-status signalling. J. Cell Sci. 122, 727–734 (2009)
S.M. Ferguson, G. Brasnjo, M. Hayashi, M. Wolfel, C. Collesi, S. Giovedi, A. Raimondi, L.W. Gong, P. Ariel, S. Paradise, E. O’Toole, R. Flavell, O. Cremona, G. Miesenbock, T.A. Ryan, P. De Camilli, A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science 316, 570–574 (2007)
M. Fine, M.C. Llaguno, V. Lariccia, M.J. Lin, A. Yaradanakul, D.W. Hilgemann, Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains. J. Gen. Physiol. 137, 137–154 (2011)
M.I. Geli, H. Riezman, Endocytic internalization in yeast and animal cells: similar and different. J. Cell Sci. 111(Pt 8), 1031–1037 (1998)
K. Hayashi, W. Shuai, Y. Sakamoto, H. Higashida, M. Yamagishi, S. Kupershmidt, Trafficking-competent KCNQ1 variably influences the function of HERG long QT alleles. Heart Rhythm 7, 973–980 (2010)
D.W. Hilgemann, M. Fine, Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains. J. Gen. Physiol. 137, 155–172 (2011)
D.W. Hilgemann, G.A. Langer, Transsarcolemmal calcium movements in arterially perfused rabbit right ventricle measured with extracellular calcium-sensitive dyes. Circ. Res. 54, 461–467 (1984)
D.W. Hilgemann, A. Yaradanakul, Y. Wang, D. Fuster, Molecular control of cardiac sodium homeostasis in health and disease. J. Cardiovasc. Electrophysiol. 17(Suppl 1), S47–S56 (2006)
T.J. Hund, P.J. Mohler, Ankyrin-based targeting pathway regulates human sinoatrial node automaticity. Channels (Austin) 2, 404–406 (2008)
A.I. Ivanov, Pharmacological inhibition of endocytic pathways: is it specific enough to be useful? Methods Mol. Biol. 440, 15–33 (2008)
S.H. Kim, T.A. Ryan, Synaptic vesicle recycling at CNS synapses without AP-2. J. Neurosci. 29, 3865–3874 (2009)
V. Lariccia, M. Fine, S. Magi, M.J. Lin, A. Yaradanakul, M.C. Llaguno, D.W. Hilgemann, Massive calcium-activated endocytosis without involvement of classical endocytic proteins. J. Gen. Physiol. 137, 111–132 (2011)
S.P. Lawrence, G.D. Holman, F. Koumanor, Translocation of the Na/H exchanger (NHE1) in cardiomyocyte responses to insulin and energy status signaling. Biochem. J. 432, 515–523 (2010)
S. Mayor, R.E. Pagano, Pathways of clathrin-independent endocytosis. Nat. Rev. Mol. Cell Biol. 8, 603–612 (2007)
G. Miesenbock, D.A. De Angelis, J.E. Rothman, Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192–195 (1998)
O. Pongs, Ins and outs of cardiac voltage-gated potassium channels. Curr. Opin. Pharmacol. 9, 311–315 (2009)
J.E. Saffitz, J.G. Laing, K.A. Yamada, Connexin expression and turnover: implications for cardiac excitability. Circ. Res. 86, 723–728 (2000)
K. Sato, G.G. Ernstrom, S. Watanabe, R.M. Weimer, C.H. Chen, M. Sato, A. Siddiqui, E.M. Jorgensen, B.D. Grant, Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools. Proc. Natl. Acad. Sci. U. S. A. 106, 1139–1144 (2009)
A. Zorzano, L. Sevilla, M. Camps, C. Becker, J. Meyer, H. Kammermeier, P. Munoz, A. Guma, X. Testar, M. Palacin, J. Blasi, Y. Fischer, Regulation of glucose transport, and glucose transporters expression and trafficking in the heart: studies in cardiac myocytes. Am. J. Cardiol. 80, 65A–76A (1997)
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Hilgemann, D.W., Lin, MJ., Fine, M., Frazier, G., Wang, HR. (2013). Toward an Understanding of the Complete NCX1 Lifetime in the Cardiac Sarcolemma. In: Annunziato, L. (eds) Sodium Calcium Exchange: A Growing Spectrum of Pathophysiological Implications. Advances in Experimental Medicine and Biology, vol 961. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-4756-6_29
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