Abstract
Hypoxanthine-guanine phosphoribosyl transferase (HGPRTase.) from |35S|-methionyl-labelled rat hepatoma (HTC) cells has been purified more than 600-fold to near homogeneity by making use of conventional and affinity chromatographic techniques (Table 1). The conventional purification procedure utilizes a pH 5.0 precipitation, an ammonium sulfate precipitation, DEAE-cellulose chromatography, and heating at 80–85°according to the method of Olsen and Milman, 1974. Using this procedure, a partially pure enzyme preparation is obtained. Affinity chromatography according to Hughes et al., 1975, can be used either before or after the DEAE step to yield pure enzyme.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Chasin, L.A. and Urlaub, G. (1976) Somatic Cell Genetics 2, 453.
Graf, L.H., McRoberts, J.A., Harrison, T. and Martin, D.W. (1976) J. Cell. Physiol. 80, 331.
Hughes, S.H., Wahl, M. and Capecchi, M.R. (1975) J. Biol. Chem. 250, 120.
O’Farrell, P.H. (1975) J. Biol. Chem. 250, 4007.
Olsen, A.S. and Milman, G. (1974) J. Biol. Chem. 249, 4030.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1977 Plenum Press, New York
About this chapter
Cite this chapter
Zannis, V.I., Gudas, L.J., Doyle, D., Martin, D.W. (1977). Purification and Characterization of Hypoxanthine-Guanine Phosphoribosyl Transferase from Cultured HTC Cells. In: Müller, M.M., Kaiser, E., Seegmiller, J.E. (eds) Purine Metabolism in Man—II. Advances in Experimental Medicine and Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4223-6_21
Download citation
DOI: https://doi.org/10.1007/978-1-4613-4223-6_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4225-0
Online ISBN: 978-1-4613-4223-6
eBook Packages: Springer Book Archive