Abstract
We examined the in vitro effects of ethyl alcohol and a recombinant HIV-1, fusion peptide, env-gag, corresponding to portions of the gp-41 envelope (env) and internal core (gag) proteins on the natural killer (NK) cell activities of lymphocytes from healthy donors. Preculture of large granular lymphocytes (LGL) from normal donors with 0.3% alcohol for 72 hr significantly inhibited NK activity compared to untreated controls. Direct addition, without preculture, of alcohol (0.1%, 0.2% and 0.3%) and env-gag peptide (10 ng/ml) at concentrations which did not affect NK activity when either was added alone, produced a significant, synergistic, inhibitory effect on NK activity when added together. This synergistic effect of alcohol and HIV-derived soluble products is consistent with a model proposing that alcohol abuse may be a co-factor involved in the progression of HIV infections.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Bagasra O, Balla AK, Lischner HW. Effects of alcohol ingestion on in vitro susceptibility of peripheral blood mononuclear cells to infection with HIV and of selected T cell functions. Alcoholism: Clinical and Experimental Research. 1989; 13: 636–643.
Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation and of granulcoytes by combining centrifugation and sedimentation. Clin. lab. Invest. 1968; 21(suppl) 97: 77.
Centers for Disease Control, MS Department of Health and Human Services. Morb. Mort. Wkly. Report. 1986; 35: 76–78.
Jerrells TR, Perin D, Marietta C and Eckardt MJ. Mechanisms of suppression of cellular immunity induced by ethanol. Alcoholism Clin. Exp. Res. 1989; 13: 490–493.
MacGregor RR. Alcohol and drugs as cofactors for AIDS. Advan. Alcohol. Sub. Abuse. 1987; 7 (2): 47–71.
Nair MPN, Pottathil R, Heimer EP, Schwartz SA. Immunoregulatory activities of human immunodeficiency virus (HIV) proteins: Effect of HIV recombinant and synthetic peptides on immunoglobulin synthesis and proliferative responses by normal lymphocytes. Proc. Natl. Acad. Sci. USA 1988; 85: 6498–6502.
Nair MPN, Schwartz SA, Kronfol ZA, Heimer EP, Pottahil R. Inimunoregulatory activities of human immunodeficiency virus (HIV) proteins. Inhibitory effect on natural killer (NK) cell functions (Abstract). 7th Intnatl Cong of Immunology, Berlin, Germany, 1989.
Nair MPN, Kronfol ZA, Schwartz SA. Effect of alcohol and nicotine on cytotoxic responses of human lymphocytes. Clin. Immunol. Immunopathol. 1990; 54: 395–409.
Pollack ES, Nomura AM, Heilburn LK, Stemmermann GN, Green SB. Prospective study of alcohol consumption and cancer. N. Engl. J. Med. 1984; 310: 617–621.
Rosenberg ZF, Fauci AS. Immunopathogenic mechanisms of HIV infection. Clin. Immunol. Immunopathol. 1989; 50: 5149–5156.
Voth R, Rossol S, Graff E, Laubenstein RHP, Schroder HC, Muller WE, Meyer zum Buschenfelde KH, Hess G. Natural killer cell activity as a prognostic parameter in the progression to AIDS. J. Infect. Dis. 1988; 157: 851.
Watson RR, Prabhala RH, Darban HR, Tahya MD, Smith TL. Changes in lymphocyte and monophages subset due to morphin and ethanol treatment during retrovirus infection causing murine AIDS. Life Sciences. 1988; 43: 5–11.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Plenum Press, New York
About this chapter
Cite this chapter
Nair, M.P.N., Schwartz, S.A. (1996). Immunopathogenesis of HIV Infection. In: Friedman, H., Eisenstein, T.K., Madden, J., Sharp, B.M. (eds) AIDS, Drugs of Abuse, and the Neuroimmune Axis. Advances in Experimental Medicine and Biology, vol 402. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0407-4_22
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0407-4_22
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-8038-2
Online ISBN: 978-1-4613-0407-4
eBook Packages: Springer Book Archive