Abstract
Recent evidence in the literature suggests that tissues play a greater role than blood in reducing nitrite to NO under ischemic or hypoxic conditions. Our previous mathematical model for coupled NO and O2 transport around an arteriole, modified to include superoxide generation from dysfunctional endothelium, was developed further to include nitrite reductase activity in blood and tissue. Steady-state radial and axial NO and pO2 profiles in the arteriole and surrounding tissue were simulated for different blood flow rates and arterial blood pO2 values. The resulting computer simulations demonstrate that nitrite reductase activity in blood is not a very effective mechanism for conserving NO due to the strong scavenging of NO by hemoglobin. In contrast, nitrite reductase activity in tissue is much more effective in increasing NO bioavailability in the vascular wall and contributes progressively more NO as tissue hypoxia becomes more severe.
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References
Buerk DG (2009) Mathematical modeling of the interaction between oxygen, nitric oxide and superoxide. Adv Exp Med Biol 645:7–12
Buerk DG, Lamkin-Kennard K, Jaron D (2003) Modeling the influence of superoxide dismutase on superoxide and nitric oxide interactions, including reversible inhibition of oxygen consumption. Free Radic Biol Med 34:1488–1503
Chen K, Piknova B, Pittman RN et al (2008) Nitric oxide from nitrite reduction by hemoglobin in the plasma and erythrocytes. Nitric Oxide 18:47–60
Chen X, Buerk DG, Barbee KA et al (2007) A model of NO/O2 transport in capillary-perfused tissue containing an arteriole and venule pair. Ann Biomed Eng 35:517–529.
Chen X, Jaron D, Barbee KA et al (2006) The influence of radial RBC distribution, blood velocity profiles, and glycocalyx on coupled NO/O2 transport. J Appl Physiol 100:482–492
Deisen DL, Hess DT, Stamler JS (2008) Hypoxic vasodilation by red blood cells: evidence for an S-nitrosothiol based signal. Circ Res 103:545–553
Feelisch M, Fernandez BO, Bryan NS et al (2008) Tissue processing of nitrite in hypoxia: an intricate interplay of nitric oxide-generating and -scavenging systems. J Biol Chem 283: 33927–33934
Gautier C, van Faassen E,Mikula I et al (2006) Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia. Biochem Biophys Res Commun 341:816–821
GladwinMT, Grubina R, Doyle MP (2009) The new chemical biology of nitrite reactions with hemoglobin: R-state catalysis, oxidative denitrosylation, and nitrite reductase/anhydrase. Acc Chem Res 42:157–167
Isbell TS, Sun C-W, Wu L-C et al (2008) SNO-hemoglobin is not essential for red blood cell-dependent hypoxic vasodilation. Nature Med 14:773–777
Li H, Cui H, Kundu TK et al (2008) Nitric oxide production from nitrite occurs primarily in tissues not in the blood: critical role of xanthine oxidase and aldehyde oxidase. J Biol Chem 283:17855–17863
van Faassen EE, Bahrami S, Feelisch M et al (2009) Nitrite as regulator of hypoxic signaling in mammalian physiology. Med Res Rev 29:683–741
Vitturi DA, Teng X, Toledo JC et al (2009) Regulation of nitrite transport in red blood cells by hemoglobin oxygen fractional saturation. Am J Physiol Heart Circ Physiol 296:H1398-H1407
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Buerk, D.G., Barbee, K.A., Jaron, D. (2011). Modeling O2-Dependent Effects of Nitrite Reductase Activity in Blood and Tissue on Coupled NO and O2 Transport around Arterioles. In: LaManna, J., Puchowicz, M., Xu, K., Harrison, D., Bruley, D. (eds) Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol 701. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7756-4_36
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DOI: https://doi.org/10.1007/978-1-4419-7756-4_36
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