Abstract
The purpose of this study was to ascertain if effects of halothane and sevoflurane (0.18–1.45 MAC) on the magnitude of the rise in intracellular calcium ([Ca2+]i with ∼90s hypoxia (measured using indo-1 dye) in rat pup carotid body type I glomus cells. paralleled their known effects on the human hypoxic ventilatory response, where halothane is more depressive. We also assessed these agents’ effect on [Ca2+]i response to 100 mM K+. Halothane depressed the [Ca2+]i transient in hypoxia more than sevoflurane (p = 0.036). Both agents also depressed the [Ca2+]i response to K+ – halothane more than sevoflurane (p = 0.004). These actions reflect their known influence on human hypoxic ventilatory response, consistent with the notion that the cellular process underlies the whole-body effect. The responses to K+, which depolarises the cell membrane, indicates that in addition to a putative effect on K+ channels, voltage-activated Ca2+ channels may also be involved in the anaesthetic effect.
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Buckler, K.J., Williams, B.A., and Honore, E. (2000) An oxygen-, acid- and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells. J. Physiol. 525, 135–142.
Cotten, J.F. and Miller, K.W. (2006) Volatile anesthetic regulation of TASK tandem pore potassium channels. Anesthesiology 105, A170.
Dolphin, A.C. (2006) A short history of voltage-gated calcium channels. Br. J. Pharmacol. 147(Suppl. 1), S56–S62.
Overholt, J.L. and Prabakhar, N. (1997) Ca2+ current in rabbit carotid body glomus cells is conducted by multiple types of high-voltage-activated Ca2+ channels. J. Neurophysiol. 78, 2467–2474.
Pandit, J.J. (2002) The variable effect of low-dose volatile anaesthetics on the acute ventilatory response to hypoxia in humans: A quantitative review. Anaesthesia 57, 632–643.
Pandit, J.J. (2007) Volatile anesthetics and the hypoxic ventilatory response: Effects, clinical implications, and future research. Sem. Anesth. Periop. Med. Pain 26, 49–57.
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Pandit, J.J., Buckler, K.J. (2010). Halothane and Sevoflurane Exert Different Degrees of Inhibition on Carotid Body Glomus Cell Intracellular Ca2+ Response to Hypoxia. In: Homma, I., Onimaru, H., Fukuchi, Y. (eds) New Frontiers in Respiratory Control. Advances in Experimental Medicine and Biology, vol 669. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5692-7_40
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DOI: https://doi.org/10.1007/978-1-4419-5692-7_40
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