Abstract
Breathing is controled by neural networks of the pre-Bötzinger complex (preBötC). We have previously established that multiphoton/confocal Ca2+ imaging is a potent tool for studying preBötC functions in transversal newborn rat brainstem slices. Here, we used such imaging to show that only 3 often dispersedly located preBötC neurons are typically inspiratory active per transversal imaging plane in slices with rostrally-exposed preBötC contrary to rhythmic optical activity in 11 densely aggregated neurons in slices with the preBötC at the caudal margin. In both slice types, glutamate raises Ca2+ in >30 cells (both neurons and glia). Factors are discussed that may be involved in the spatial inhomogeneity of superficially located active inspiratory preBötC neurons in both slice types.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ballanyi, K. and Ruangkittisakul, A. (2009) Structure-function analysis of rhythmogenic inspiratory pre-Bötzinger complex networks in “calibrated” newborn rat brainstem slices. Respir. Physiol. Neurobiol. 168, 158–178.
Barnes, B.J., Tuong, C.M., and Mellen, N.M. (2007) Functional imaging reveals respiratory network activity during hypoxic and opioid challenge in the neonate rat tilted sagittal slab preparation. J. Neurophysiol. 97, 2283–2292.
Funke, F., Dutschmann, M., and Müller, M. (2007) Imaging of respiratory-related population activity with single-cell resolution. Am. J. Physiol. Cell. Physiol. 292, C508–C516.
Hayes, J.A. and Del Negro, C.A. (2007) Neurokinin receptor-expressing pre-botzinger complex neurons in neonatal mice studied in vitro. J. Neurophysiol. 97, 4215–4224.
Koshiya, N. and Smith, J.C. (1999) Neuronal pacemaker for breathing visualized in vitro. Nature 400, 360–363.
Ruangkittisakul, A., Okada, Y., Oku, Y., Koshiya, N., and Ballanyi, K. (2009) Fluorescence imaging of active respiratory networks. Respir. Physiol. Neurobiol. 168, 26–38.
Ruangkittisakul, A., Schwarzacher, S.W., Secchia, L., Poon, B.Y., Ma, Y., Funk, G.D., and Ballanyi, K. (2006) High sensitivity to neuromodulator-activated signalling pathways at physiological [K+] of confocally-imaged respiratory centre neurons in online-calibrated newborn rat brainstem slices. J. Neurosci. 26, 11870–11880.
Ruangkittisakul, A., Schwarzacher, S.W., Secchia, L., Ma, Y., Bobocea, N., Poon, B.Y., Funk, G.D., and Ballanyi, K. (2008) Generation of eupnea and sighs by a spatiochemically organized inspiratory network. J. Neurosci. 28, 2447–2458.
Acknowledgements
Supported by CIHR, AHFMR, CFI and ISRIP-ASRA. KB is AHFMR Scientist. AR was supported by CIHR studentship (MFN training grant), NB by Center for Neuroscience (UofA).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this paper
Cite this paper
Bobocea, N., Ruangkittisakul, A., Ballanyi, K. (2010). Multiphoton/Confocal Ca2+-Imaging of Inspiratory pre-Bötzinger Complex Neurons at the Rostral or Caudal Surface of Newborn Rat Brainstem Slices. In: Homma, I., Onimaru, H., Fukuchi, Y. (eds) New Frontiers in Respiratory Control. Advances in Experimental Medicine and Biology, vol 669. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5692-7_17
Download citation
DOI: https://doi.org/10.1007/978-1-4419-5692-7_17
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4419-5691-0
Online ISBN: 978-1-4419-5692-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)