Aspartame (Apt), Acesulfame-K (Ace-K) low-calorie, high-potency artificial sweeteners are currently used in beverages and dietary food and drinks. Their increased application in food and drink products has given a new impetus to develop fast and accurate methods for their determination. Absorption spectra of Asp, Caf, Ace-K and BA strongly overlap. Therefore a direct determination of these analytes in quaternary mixture is impossible without a separation step. In order to overcome this difficulty partial least squares (PLS) method has been proposed.
A spectrophotometric method for the routine, rapid end simultaneous determination of Ace-K, Caf, Aspt and BA in cola drinks was investigated. A calibration set containing between 3 – 5 μg mL−1 of Ace-K, Caf, and BA, 5 – 7 μg mL−1 of Aspt was used.
The method involves the use of 27 standard mixtures of the four compounds assayed, considered at three concentration levels and measured of samples in a 0.1 N H 3 PO 4 . The designed and optimized training set of calibration was applied to the determination of four food additives in several synthetic mixtures. Three commercial cola drinks (coca-cola, pepsi-cola, cola-turka) that contained the four additives were also satisfactorily analyzed without separation step.
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Turak, F., Özgür, M.Ü., Bozdoğan, A. (2009). Simultaneous Spectrophotometric Determination of Food Additives (Benzoic Acid, Caffeine, Aspartame and Acesulfame-K) in Cola Drinks by PLS Multivariate Calibration Method. In: Şener, B. (eds) Innovations in Chemical Biology. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6955-0_32
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DOI: https://doi.org/10.1007/978-1-4020-6955-0_32
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