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A New Affinity-Based Probe to Profile MMP Active Forms

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Proteases and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2747))

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Abstract

A new generation of affinity-based probes (AfBPs) has been developed to label and identity matrix metalloproteinases (MMPs) under their active form in complex proteomes. First, the probe reacts with an active MMP through a proximity-driven reaction that does not require any external trigger. Following this affinity-labeling step, a streptavidin-based enrichment of the resulting biotin-tagged MMP is carried out. Finally, after on-beads proteolytic digestion by trypsin, MMP signature peptides are analyzed and identified by mass spectrometry. Such a “photoactivation-free” labeling can be applied to the detection of several MMPs in a wide variety of biological systems, including in vivo conditions.

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Acknowledgments

This work was supported by the French National Research Agency (ANR-18-CE44-0012).

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Authors and Affiliations

  1. Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SIMoS, Gif-sur-Yvette, France

    Carole Malgorn, Pierrick Bruyat, Carole Fruchart-Gaillard, Fabrice Beau, Sarah Bregant & Laurent Devel

  2. Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Gif-sur-Yvette, France

    François Becher

Authors
  1. Carole Malgorn
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  2. François Becher
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  3. Pierrick Bruyat
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  4. Carole Fruchart-Gaillard
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  5. Fabrice Beau
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  6. Sarah Bregant
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  7. Laurent Devel
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Corresponding author

Correspondence to Laurent Devel .

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Editors and Affiliations

  1. Department of Biochemical Sciences, School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK

    Salvatore Santamaria

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Malgorn, C. et al. (2024). A New Affinity-Based Probe to Profile MMP Active Forms. In: Santamaria, S. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 2747. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3589-6_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3589-6_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3588-9

  • Online ISBN: 978-1-0716-3589-6

  • eBook Packages: Springer Protocols

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