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Histochemical Detection of Peroxidase and Laccase Activities in Populus Secondary Xylem

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Xylem

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2722))

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Abstract

Peroxidases (PRXs) and laccases (LACs) are enzymes involved in catalyzing the oxidation of the lignin monomers to facilitate lignin polymerization. However, due to the large number of genes composing these two families of enzymes, many details regarding their specific localization are only partially understood. Here, we present a fast and easy histochemical method that makes use of the artificial substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to visualize PRX and LAC activities in the hybrid aspen (Populus tremula x P. tremuloides) xylem tissue. In addition, we describe a protocol that allows the detection of the PRX substrate, H2O2, using the nonfluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) in woody tissues.

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Authors and Affiliations

  1. Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden

    Marta-Marina Pérez Alonso, Àngela Carrió-Seguí & Hannele Tuominen

Authors
  1. Marta-Marina Pérez Alonso
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  2. Àngela Carrió-Seguí
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  3. Hannele Tuominen
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Corresponding author

Correspondence to Hannele Tuominen .

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Editors and Affiliations

  1. UPV-CSIC, Universitat Politècnica de València, Valencia, Spain

    Javier Agusti

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Alonso, MM.P., Carrió-Seguí, À., Tuominen, H. (2024). Histochemical Detection of Peroxidase and Laccase Activities in Populus Secondary Xylem. In: Agusti, J. (eds) Xylem. Methods in Molecular Biology, vol 2722. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3477-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3477-6_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3476-9

  • Online ISBN: 978-1-0716-3477-6

  • eBook Packages: Springer Protocols

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