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Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites

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SH2 Domains

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2705))

Abstract

Protein engineering has brought advances to industrial processes, biomaterials, nanotechnology, biosensors, and biomedical applications. This chapter will focus on the engineering of Src Homology 2 domains (SH2) to act as an antibody mimetic for the recognition of sulfotyrosine-containing peptides or proteins. In comparison to anti-sulfotyrosine antibodies, SH2 mutants have much smaller size and can be heterologously expressed and purified in large quantity at low cost. This chapter will describe the use of phage display to identify a sulfotyrosine-binding SH2 mutant and the subsequent enrichment of sulfotyrosine-containing peptides in complex biological samples.

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Acknowledgments

This work was supported by National Institute of Health (grant 1R01GM138623 and 1R01GM147785 to J.G. and W.N.).

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA

    Sean Paul Waldrop & Jiantao Guo

  2. Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA

    Wei Niu

  3. The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, NE, USA

    Wei Niu & Jiantao Guo

Authors
  1. Sean Paul Waldrop
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  2. Wei Niu
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  3. Jiantao Guo
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Corresponding author

Correspondence to Jiantao Guo .

Editor information

Editors and Affiliations

  1. School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK

    Teresa Carlomagno

  2. Signalling Research Centres BIOSS &CIBSS, University of Freiburg, Freiburg, Germany

    Maja Köhn

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Waldrop, S.P., Niu, W., Guo, J. (2023). Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites. In: Carlomagno, T., Köhn, M. (eds) SH2 Domains. Methods in Molecular Biology, vol 2705. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3393-9_16

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  • DOI: https://doi.org/10.1007/978-1-0716-3393-9_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3392-2

  • Online ISBN: 978-1-0716-3393-9

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