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MALDI and Trace Metal Analysis in Age-Related Macular Degeneration

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Imaging Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2688))

Abstract

Age-related macular degeneration (AMD) remains one of the most prevalent causes of blindness throughout the world. Key to prevention of AMD is furthering the understanding of its pathology. In recent years, both the proteins within the innate immune system and essential and non-essential metals have been implicated in the pathology of AMD. Herein, a multidisciplinary and multimodal methodology has been taken to further our understanding of the role of the innate immune proteins and the essential metals within mouse ocular tissue.

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References

  1. Wong WL, Su X, Li X, Cheung CM, Klein R, Cheng CY, Wong TY (2014) Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis. The Lancet. Global health, 2(2), e106–e116. https://doi.org/10.1016/S2214-109X(13)70145-1

  2. Papadopoulos Z (2020) Recent developments in the treatment of wet age-related macular degeneration. Curr Med Sci 40:851–857. https://doi.org/10.1007/s11596-020-2253-6

    Article  PubMed  Google Scholar 

  3. Rayess N, Houston SKS, Gupta OP, Ho AC, Regillo CD (2015) Treatment outcomes after 3 years in neovascular age-related macular degeneration using a treat-and-extend regimen. Am J Ophthalmol 159:3–8.e1. https://doi.org/10.1016/j.ajo.2014.09.011

    Article  PubMed  Google Scholar 

  4. Danis RP, Lavine JA, Domalpally A (2015) Geographic atrophy in patients with advanced dry age-related macular degeneration: current challenges and future prospects. Clinical Ophthalmology (Auckland, N.Z.) 9:2159–2174. https://doi.org/10.2147/OPTH.S92359

    Article  CAS  PubMed  Google Scholar 

  5. Doyle S, Mulfaul K, Fernando N, Chirco K, Connolly E, Ryan T, Ozaki E, Brennan K, Maminishkis A, Salomon R (2018) Invest Ophthalmol Vis Sci 59:3475

    Google Scholar 

  6. Ozaki E, Gibbons L, Neto NG, Kenna P, Carty M, Humphries M, Humphries P, Campbell M, Monaghan M, Bowie A, Doyle SL (2020) SARM1 deficiency promotes rod and cone photoreceptor cell survival in a model of retinal degeneration. Life Science Alliance 3:e201900618. https://doi.org/10.26508/lsa.201900618

    Article  PubMed  PubMed Central  Google Scholar 

  7. Mulfaul K, Ozaki E, Fernando N, Brennan K, Chirco KR, Connolly E, Greene C, Maminishkis A, Salomon RG, Linetsky M, Natoli R, Mullins RF, Campbell M, Doyle SL (2020) Toll-like receptor 2 facilitates oxidative damage induced retinal degeneration. Cell Reports (Cambridge) 30:2209–2224.e5. https://doi.org/10.1016/j.celrep.2020.01.064

    Article  CAS  Google Scholar 

  8. Sarah LD, Campbell M, Ozaki E, Robert GS, Mori A, Paul FK, Gwyneth JF, Kiang A-s, Marian MH, Ed CL, Luke AJO, Joe GH, Humphries P (2012) Nat Med 18:791–798. https://doi.org/10.1038/nm.2717

    Article  CAS  Google Scholar 

  9. Tarallo V, Hirano Y, Gelfand B, Dridi S, Kerur N, Kim Y, Cho W, Kaneko H, Fowler B, Bogdanovich S, Albuquerque RC, Hauswirth W, Chiodo V, Kugel J, Goodrich J, Ponicsan S, Chaudhuri G, Murphy M, Dunaief J, Ambati B, Ambati J, Ogura Y, Yoo J, Lee D, Provost P, Hinton D, Núñez G, Baffi J, Kleinman M (2012) Cell 149:847–859. https://doi.org/10.1016/j.cell.2012.03.036

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG (2018) Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration. Sci Rep 8:461–412. https://doi.org/10.1038/s41598-017-17634-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Seeley EH, Oppenheimer SR, Mi D, Chaurand P, Caprioli RM (2008) J Am Soc Mass Spectrom 19:1069–1077. https://doi.org/10.1016/j.jasms.2008.03.016

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Rashid K, Akhtar-Schaefer I, Langmann T (2019) Front Immunol 10:1975. https://doi.org/10.3389/fimmu.2019.01975

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Norris JL, Caprioli RM (2013) Chem Rev 113:2309–2342. https://doi.org/10.1021/cr3004295

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Limbeck A, Galler P, Bonta M, Bauer G, Nischkauer W, Vanhaecke F (2015) Anal Bioanal Chem 407:6593–6617. https://doi.org/10.1007/s00216-015-8858-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Šala M, Šelih VS, van Elteren JT (2017) Analyst (London) 142:3356–3359. https://doi.org/10.1039/C7AN01361B

    Article  Google Scholar 

  16. López-Fernández H, de Pessôa GS, Arruda MAZ, Capelo-Martínez JL, Fdez-Riverola F, Glez-Peña D, Reboiro-Jato M (2016) J Cheminform 8:65. https://doi.org/10.1186/s13321-016-0178-7

    Article  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Joshua Millar, Susan Campbell, Catherine Duckett & Laura M. Cole

  2. Immunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuro-science (TCIN), School of Medicine, Trinity College Dublin (TCD), Dublin, Ireland

    Sarah Doyle

Authors
  1. Joshua Millar
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  2. Susan Campbell
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  3. Catherine Duckett
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  4. Sarah Doyle
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  5. Laura M. Cole
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Corresponding author

Correspondence to Laura M. Cole .

Editor information

Editors and Affiliations

  1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Laura M Cole

  2. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Malcolm R Clench

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Millar, J., Campbell, S., Duckett, C., Doyle, S., Cole, L.M. (2023). MALDI and Trace Metal Analysis in Age-Related Macular Degeneration. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3319-9_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3318-2

  • Online ISBN: 978-1-0716-3319-9

  • eBook Packages: Springer Protocols

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