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Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish

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Axon Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2636))

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Abstract

Axon severing results in diverse outcomes, including successful regeneration and reestablishment of function, failure to regenerate, or neuronal cell death. Experimentally injuring an axon makes it possible to study degeneration of the distal stump that was detached from the cell body and document the successive steps of regeneration. Precise injury reduces damage to the environment surrounding an axon, and thereby the involvement of extrinsic processes, such as scarring or inflammation, enabling researchers to isolate the role that intrinsic factors play in regeneration. Several methods have been used to sever axons, each with advantages and disadvantages. This chapter describes using a laser on a two-photon microscope to cut individual axons of touch-sensing neurons in zebrafish larvae, and live confocal imaging to monitor its regeneration, a method that provides exceptional resolution.

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Acknowledgments

KPA was supported by a Cota-Robles fellowship, Bridge to the Doctorate Fellowship, and NIH fellowship F31NS106742-02. This work was supported by NIH grant R01AR064582 (to AS).

Author information

Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA

    Kadidia P. Adula & Alvaro Sagasti

Authors
  1. Kadidia P. Adula
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  2. Alvaro Sagasti
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Corresponding author

Correspondence to Alvaro Sagasti .

Editor information

Editors and Affiliations

  1. Department of Biology, Appalachian State University, Boone, NC, USA

    Ava J. Udvadia

  2. Office of Technology Development, Medical College of Wisconsin, Milwaukee, WI, USA

    James B. Antczak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Adula, K.P., Sagasti, A. (2023). Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish. In: Udvadia, A.J., Antczak, J.B. (eds) Axon Regeneration. Methods in Molecular Biology, vol 2636. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3012-9_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3012-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3011-2

  • Online ISBN: 978-1-0716-3012-9

  • eBook Packages: Springer Protocols

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