Abstract
The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.
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Acknowledgments
This work was supported by the Office of Research Infrastructure Programs, Office of the Director, of the National Institutes of Health under award numbers P40 OD011062 (ECB) and R43/44OD018231 (BJS). In addition, the NSET technology was supported in part by an award from the Kentucky Cabinet for Economic Development, Office of Commercialization and Innovation under the grant agreement KSTC-184-512-10-096 with the Kentucky Science and Technology Corporation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or other funding agencies.
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Bryda, E.C., Men, H., Stone, B.J. (2023). Rat Embryonic Stem Cell Transgenesis. In: Saunders, T.L. (eds) Transgenesis. Methods in Molecular Biology, vol 2631. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2990-1_16
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DOI: https://doi.org/10.1007/978-1-0716-2990-1_16
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2990-1
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