Abstract
Flow cytometry is a fluorescence-based technology that allows for the identification and characterization of immune cell subsets within a heterogenous population. Briefly, isolated immune cells are stained in suspension with fluorescently tagged antibodies to identify cells of interest prior to being run through a flow cytometer. Here we describe how to isolate murine immune cells from various body regions, including the inguinal lymph nodes (ILNs), spleen, thymus, and peripheral blood, and tag them with primary fluorescent antibodies for flow cytometric analysis of CD4+ and CD8+ T cell populations. This chapter also details how to use flow cytometry to measure T cell expression of chemokine receptor 7 (CCR7), the major chemokine receptor lymphocytes use to enter lymph nodes. The methods described in this chapter can be used for characterizing other proteins of interest, as well as other immune cell subsets.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Manhas, K.R., Blattman, J.N. (2023). Flow Cytometry Analysis of Immune Cell Responses. In: Lucas, A.R. (eds) Chemokine-Glycosaminoglycan Interactions. Methods in Molecular Biology, vol 2597. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2835-5_9
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DOI: https://doi.org/10.1007/978-1-0716-2835-5_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2834-8
Online ISBN: 978-1-0716-2835-5
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