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Noninvasive Quantitative PET Imaging in Humans of the Pancreatic Beta-Cell Mass Biomarkers VMAT2 and Dopamine D2/D3 Receptors In Vivo

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2592))

Abstract

Noninvasive quantitative imaging of beta-cells can provide information on changes in cellular transporters, receptors, and signaling proteins that may affect function and/or loss of mass, both of which contribute to the loss of insulin secretion and glucose regulation of patients with type 1 or type 2 diabetes (T1D/T2D). We have developed and optimized the use of two positron emission tomography (PET) radioligands, [18F]FP-(+)-DTBZ and [11C](+)-PHNO, targeting beta-cell VMAT2 and dopamine (D2/D3) receptors, respectively. Here we describe our optimized methodology for the clinical use of these two tracers for quantitative PET imaging of beta-cell biomarkers in vivo. We also briefly discuss our previous results and their implications and value towards extending the use of PET radioligand beyond the original goal of quantitative imaging of beta-cell mass to the potential to provide insight into the biology of beta-cell loss of mass and/or function and to evaluate the efficacy of therapeutics to prevent or restore functional beta-cell mass.

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Acknowledgements

This work was supported by a training grant from the NIDDK (K01DK118005).

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Authors and Affiliations

  1. PET Center, Yale University School of Medicine, New Haven, CT, USA

    Jason Bini & Richard E. Carson

  2. Department of Internal Medicine, Division of Endocrinology, Yale University School of Medicine, New Haven, CT, USA

    Gary W. Cline

Authors
  1. Jason Bini
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  2. Richard E. Carson
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  3. Gary W. Cline
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Corresponding author

Correspondence to Jason Bini .

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Editors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Anna Moore

  2. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Ping Wang

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Bini, J., Carson, R.E., Cline, G.W. (2023). Noninvasive Quantitative PET Imaging in Humans of the Pancreatic Beta-Cell Mass Biomarkers VMAT2 and Dopamine D2/D3 Receptors In Vivo. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2807-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2806-5

  • Online ISBN: 978-1-0716-2807-2

  • eBook Packages: Springer Protocols

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