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Assay of Endocannabinoid Oxidation by Cytochrome P450

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2576))

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Abstract

Cytochrome P450 enzymes are a large family of heme-containing proteins that have important functions in the biotransformation of xenobiotics, including pharmacologic and environmental agents, as well as endogenously produced chemicals with broad structural and functional diversity. Anandamide and 2-arachidonoylglycerol (2-AG) are substrates for P450s expressed in multiple tissues, leading to the production of a diverse set of mono- and di-oxygenated metabolites. This chapter describes tools and methods that have been used to identify major endocannabinoid metabolizing P450s and their corresponding products using subcellular tissue fractions, cultured cells, and purified recombinant enzymes in a reconstituted system.

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Acknowledgments

This work was supported in part by the National Institutes of Health Grants CA16954, GM007767 and DK110355.

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Authors and Affiliations

  1. Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Natasha T. Snider

  2. Department of Pharmacology, University of Michigan, Ann Arbor, MI, USA

    Paul F. Hollenberg

Authors
  1. Natasha T. Snider
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  2. Paul F. Hollenberg
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Corresponding author

Correspondence to Natasha T. Snider .

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Editors and Affiliations

  1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Mauro Maccarrone

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Snider, N.T., Hollenberg, P.F. (2023). Assay of Endocannabinoid Oxidation by Cytochrome P450. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_27

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  • DOI: https://doi.org/10.1007/978-1-0716-2728-0_27

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2727-3

  • Online ISBN: 978-1-0716-2728-0

  • eBook Packages: Springer Protocols

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