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In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy

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Gene, Drug, and Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2575))

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Abstract

Tumors are usually associated with oxygen-deficient regions (hypoxia) which results from reduced and disorganized intratumoral vasculature, increased diffusion distances, and growing tumor masses. The proteomic and metabolomic landscape of the hypoxic cells is reprogrammed through hypoxia-induced transcription factor 1 which is activated in hypoxic conditions and is inactive when oxygen is abundant. This transcription factor has also been shown to inhibit or even reverse cell differentiation. Hypoxia impedes chemotherapy as it hampers the formation of cytotoxic free radicals due to the lesser availability of molecular oxygen. The metastatic and invasive attributes of cancer cells in hypoxic conditions are exacerbated, which results in poor therapeutic outcomes. Various cell-based assays for measuring hypoxia have been developed which give an estimate of the hypoxic state of cancer cells. Prior knowledge of these assays will improve the efficacy of the treatment regimens for cancers. This article provides exhaustive information on the hypoxia-based assays which are sensitive, robust, reliable, and give easy readout with choice of cell type for these assays may be dictated by the procedural or endpoint selection.

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Authors and Affiliations

  1. School of Medicine, Ophthalmology, Visual and Anatomical Sciences, Wayne State University, Detroit, MI, USA

    Kartikeya Tiwari

  2. Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, USA

    Ritesh Kumar

  3. Department of Basic & Translational Sciences, Penn Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Gundappa Saha

  4. Department of Biotechnology, National Institute of Technology, Warangal, Telangana, India

    Prakash Saudagar

Authors
  1. Kartikeya Tiwari
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  2. Ritesh Kumar
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  3. Gundappa Saha
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  4. Prakash Saudagar
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Corresponding author

Correspondence to Prakash Saudagar .

Editor information

Editors and Affiliations

  1. Bioengineering Department, Imperial College London, London, UK

    Glaucia C. Pereira

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Tiwari, K., Kumar, R., Saha, G., Saudagar, P. (2023). In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy. In: Pereira, G.C. (eds) Gene, Drug, and Tissue Engineering. Methods in Molecular Biology, vol 2575. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2716-7_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2716-7_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2715-0

  • Online ISBN: 978-1-0716-2716-7

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