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Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells

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Hematopoietic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2567))

Abstract

Mobilization or egress of stem cells from bone marrow (BM) into peripheral blood (PB) is an evolutionary preserved and important mechanism in an organism for self-defense and regeneration. BM-derived stem cells circulate always at steady-state conditions in PB, and their number increases during stress situations related to (a) infections, (b) tissue organ injury, (c) stress, and (d) strenuous exercise. Stem cells also show a circadian pattern of their PB circulating level with peak in early morning hours and nadir late at night. The number of circulating in PB stem cells could be pharmacologically increased after administration of some drugs such as cytokine granulocyte colony-stimulating factor (G-CSF) or small molecular antagonist of CXCR4 receptor AMD3100 (Plerixafor) that promote their egress from BM into PB and lymphatic vessels. Circulating can be isolated from PB for transplantation purposes by leukapheresis. This important homeostatic mechanism is governed by several intrinsic complementary pathways. In this chapter, we will discuss the role of purinergic signaling and extracellular nucleotides in regulating this process and review experimental strategies to study their involvement in mobilization of various types of stem cells that reside in murine BM.

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Acknowledgments

This work was supported by NIH grants 2R01 DK074720, Stella and Henry Hoenig Endowment, OPUS grant UMO-2018/29/B/NZ4/01470 to MZR, and OPUS grant 2016/21/B/NZ4/00201 to MK. AT was supported by NIH T32 HL134644 to MZR.

Author information

Authors and Affiliations

  1. Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA

    Malwina Suszynska, Arjun Thapa, Janina Ratajczak, Magdalena Kucia & Mariusz Z. Ratajczak

  2. Department of Molecular Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland

    Malwina Suszynska

  3. Department of Regenerative Medicine, Warsaw Medical University, Warsaw, Poland

    Mateusz Adamiak, Monika Cymer, Magdalena Kucia & Mariusz Z. Ratajczak

Authors
  1. Malwina Suszynska
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  2. Mateusz Adamiak
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  3. Arjun Thapa
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  4. Monika Cymer
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  5. Janina Ratajczak
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  6. Magdalena Kucia
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  7. Mariusz Z. Ratajczak
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Corresponding author

Correspondence to Magdalena Kucia .

Editor information

Editors and Affiliations

  1. Department of Microbiology & Immunology and Department of Medicine/Hematology Oncology, Indiana University School of Medicine, Indianapolis, IN, USA

    Louis M. Pelus

  2. Moderna Therapeutics, Cambridge, MA, USA

    Jonathan Hoggatt

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Suszynska, M. et al. (2023). Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells. In: Pelus, L.M., Hoggatt, J. (eds) Hematopoietic Stem Cells. Methods in Molecular Biology, vol 2567. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2679-5_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2679-5_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2678-8

  • Online ISBN: 978-1-0716-2679-5

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