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DNA Visualization Using Fluorescent Proteins

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Fluorescent Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2564))

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Abstract

DNA binding fluorescent proteins are a powerful tool for single-molecule visualization. In this chapter, we discuss a protocol for the synthesis of DNA binding fluorescent proteins and visualization of single DNA molecules. This chapter includes stepwise methods for molecular cloning, reversible staining, two-color staining, sequence-specific staining, and microscopic visualization of single DNA molecules in a microfluidic device. This content will be useful for DNA characterization using DNA binding fluorescent proteins and its visualization at the single-molecule level.

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Acknowledgments

The authors thank Dr. SH Lee, Ms. JH Park, Ms. EJ Shin, and Ms. WJ Kim for assisting in the development of FP-DBP. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2016R1A6A1A03012845 and NRF-2020R1A2B5B02001831).

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Authors and Affiliations

  1. College of Agriculture, Yanbian University, Yanji, Jilin Province, China

    Xuelin Jin

  2. Department of Chemistry and Program of Integrated Biotechnology, Sogang University, Seoul, Korea

    Y. Tehee Kim & Kyubong Jo

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  1. Xuelin Jin
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  2. Y. Tehee Kim
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  3. Kyubong Jo
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Correspondence to Xuelin Jin or Kyubong Jo .

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Editors and Affiliations

  1. Institute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland

    Mayank Sharma

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Jin, X., Kim, Y.T., Jo, K. (2023). DNA Visualization Using Fluorescent Proteins. In: Sharma, M. (eds) Fluorescent Proteins. Methods in Molecular Biology, vol 2564. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2667-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2667-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2666-5

  • Online ISBN: 978-1-0716-2667-2

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