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Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza

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Polyploidy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2545))

Abstract

In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change.

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Acknowledgments

This work was supported by Ghent University (Methusalem funding, BOF.MET.2021.0005.01), Research Foundation Flanders (personal grants to Lucas Prost and Quinten Bafort), and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 833522).

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Authors and Affiliations

  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Tian Wu, Annelore Natran, Lucas Prost, Eylem Aydogdu, Yves Van de Peer & Quinten Bafort

  2. VIB Center for Plant Systems Biology, VIB, Ghent, Belgium

    Tian Wu, Annelore Natran, Lucas Prost, Eylem Aydogdu & Quinten Bafort

  3. Department of Biology, Ghent University, Ghent, Belgium

    Lucas Prost & Quinten Bafort

Authors
  1. Tian Wu
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  2. Annelore Natran
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  3. Lucas Prost
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  4. Eylem Aydogdu
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  5. Yves Van de Peer
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  6. Quinten Bafort
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Corresponding author

Correspondence to Quinten Bafort .

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Editors and Affiliations

  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Yves Van de Peer

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Wu, T., Natran, A., Prost, L., Aydogdu, E., Van de Peer, Y., Bafort, Q. (2023). Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza. In: Van de Peer, Y. (eds) Polyploidy. Methods in Molecular Biology, vol 2545. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2561-3_19

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  • DOI: https://doi.org/10.1007/978-1-0716-2561-3_19

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