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Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model

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Candida Species

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2542))

Abstract

The role of fungal colonizers of the gastrointestinal tract during disease states is not well understood. Antibiotic treatment renders patients highly susceptible to infection by the bacterial pathogen C. difficile while also leading to blooms in fungal commensals, setting the stage for trans-kingdom interactions. Here, we describe a murine model of Candida gastrointestinal colonization coupled to a C. difficile infection (CDI) model, the measurement of CFU of both organisms, and collection of cecum and colon contents for the purpose of quantifying C. difficile toxin production. Additionally, we describe how to induce and purify C. difficile spores.

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Acknowledgments

J.A.R is supported by the Tufts IRACDA Award Number K12GM133314 from the National Institute of General Medical Sciences. Research in the Kumamoto lab is supported by grants R01AI118898 and U19AI131126 from the National Institutes of Health.

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Authors and Affiliations

  1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA

    Jesús A. Romo & Carol A. Kumamoto

Authors
  1. Jesús A. Romo
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  2. Carol A. Kumamoto
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Corresponding author

Correspondence to Carol A. Kumamoto .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Georgetown University, Washington, DC, DC, USA

    Richard Calderone

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Romo, J.A., Kumamoto, C.A. (2022). Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model. In: Calderone, R. (eds) Candida Species. Methods in Molecular Biology, vol 2542. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2549-1_20

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  • DOI: https://doi.org/10.1007/978-1-0716-2549-1_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2548-4

  • Online ISBN: 978-1-0716-2549-1

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