Abstract
Manipulation of neural activity in genetically predefined populations of neurons through genetic techniques is an essential tool in the field of neuroscience as well as a potential avenue in treating a vast assortment of neurological and psychiatric diseases. Here, we describe an emerging methodology of molecular neuromodulation termed bioluminescence-optogenetics (BL-OG) where BL is harnessed to activate bacterial light-driven channels and pumps expressed in neurons to control their activity. BL-OG is realized through opsin-luciferase fusion proteins called luminopsins (LMOs). In this chapter, we will provide a practical guide for applying BL-OG and LMOs in vitro using a cell line and primary cells in culture. In the following chapter, we will turn our focus towards BL-OG applications in ex vivo and in vivo rodent models of the nervous system.
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Acknowledgments
Support for this project was provided by NSF CBET-1512826 (KB/REG), NIH F31NS115479 (MAS), R21NS112948 (REG), S10OD021773 (KB), DOD W81XWH1910776 (REG), and the Mirowski Family Foundation (REG).
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Stern, M.A., Skelton, H., Fernandez, A.M., Gutekunst, CA.N., Berglund, K., Gross, R.E. (2022). Bioluminescence-Optogenetics: A Practical Guide. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2525. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2473-9_26
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DOI: https://doi.org/10.1007/978-1-0716-2473-9_26
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