Abstract
RNA thermometers are RNA regulatory elements that convert temperature into a functional biological response through a temperature-induced conformational change. These regulatory elements have been investigated in numerous natural contexts and have been designed for synthetic biology as well. A basic challenge has been the design of an RNA thermometer whose final activity in response to temperature matches a prespecified response, in terms of its sensitivity, threshold, and leakiness. This chapter provides a methodology for the design of a toolbox of RNA thermometers. We describe considerations for the conceptual design, a computational assessment, and strategies for experimental synthesis and measurement.
This article is dedicated to the memory of Prof. Mashuq un Nabi.
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Acknowledgments
Support from DBT (BT/PR28513/med/32/672/2019) and the Discover and Learn Program, IRD, IIT Delhi is gratefully acknowledged.
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Sen, S., Patel, A., Gola, K.K. (2022). Design of a Toolbox of RNA Thermometers. In: Chappell, J., Takahashi, M.K. (eds) Riboregulator Design and Analysis. Methods in Molecular Biology, vol 2518. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2421-0_8
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DOI: https://doi.org/10.1007/978-1-0716-2421-0_8
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