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Optogenetic Control of Human Stem Cell-Derived Neurons

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Rhodopsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2501))

Abstract

Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and multi-electrode array (MEA) electrophysiology. Optogenetic activation of these human neurons facilitates the characterization of their functional properties at the single neuron and circuit level. Here we showcase the preparation of hiPSC-derived neurons expressing optogenetic actuators, in vitro optogenetic stimulation and simultaneous functional recordings using patch clamp and MEA electrophysiology.

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Acknowledgments

The Volkswagen Foundation (Freigeist—A110720), the European Research Council (ERC-StG 678071—ProNeurons), and the Deutsche Forschungsgemeinschaft (EXC-2151-390873048—Cluster of Excellence—ImmunoSensation2 at the University of Bonn and SPP2127) support VB. JS acknowledges the support by the Joachim Herz Stiftung.

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Authors and Affiliations

  1. Department of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany

    Rouhollah Habibey, Johannes Striebel, Kritika Sharma & Volker Busskamp

Authors
  1. Rouhollah Habibey
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  2. Johannes Striebel
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  3. Kritika Sharma
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  4. Volker Busskamp
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Corresponding author

Correspondence to Volker Busskamp .

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Editors and Affiliations

  1. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    Valentin Gordeliy

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Habibey, R., Striebel, J., Sharma, K., Busskamp, V. (2022). Optogenetic Control of Human Stem Cell-Derived Neurons. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2329-9_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2328-2

  • Online ISBN: 978-1-0716-2329-9

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