Abstract
Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.
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Acknowledgments
The authors thank J. McCann for constructive comments on the manuscript. A.J.E. received support for this project through grants from: the Breast Cancer Research Foundation (BCRF-20-048), Twisted Pink Foundation, Hope Scarves, the Jayne Koskinas Ted Giovanis Foundation, and the National Institutes of Health/National Cancer Institute (U01CA217846, U54CA2101732, 3P30CA006973). I.S.C. received support for this project through grants from: National Institutes of Health/National Cancer Institute (T32CA009071) and the Conquer Cancer Foundation Young Investigator Award. A.J.E. has unlicensed patents and patent applications on the use of 3D culture assays to identify invasive cancer cell populations, on the use of antibodies as anti-cancer therapeutics, and on NK cell-cancer cell co-cultures (w/ I.S.C.). A.J.E.’s spouse is an employee of Immunocore.
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Chan, I.S., Ewald, A.J. (2022). Organoid Co-culture Methods to Capture Cancer Cell–Natural Killer Cell Interactions. In: Shimasaki, N. (eds) Natural Killer (NK) Cells. Methods in Molecular Biology, vol 2463. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2160-8_17
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DOI: https://doi.org/10.1007/978-1-0716-2160-8_17
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2159-2
Online ISBN: 978-1-0716-2160-8
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