Abstract
Murine laser-induced laser choroidal neovascularization is a widely used and robust model of wet (exudative) age-related macular degeneration (wAMD). wAMD is one of the leading causes of blindness in the Western world. In brief, a focused laser beam is used to penetrate Bruch’s membrane, which separates the choriocapillaris (well-vascularized choroid layer) from the pigmented layers of the retina. Damage to the integrity of this membrane during diabetes leads to fluid accumulation and vascular invasion into the subretinal layers resulting in a progressive worsening of vision. Here we describe a 14-day model using untreated C57/Bl6 mice, but it is equally applicable to incorporation into transgenic studies and therapeutic agent development (such as eye drops), injection of therapeutic agents (including antibodies), and for longer time course studies. In vivo functional analysis or lesioned choroids can be studied with further immunohistochemical staining for further analyses.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gragoudas ES, Adamis AP, Cunningham ET Jr, Feinsod M, Guyer DR (2004) Pegaptanib for neovascular age-related macular degeneration. N Engl J Med 351:2805–2816
Liu C-H, Wang Z, Sun Y, Chen J (2017) Animal models of ocular angiogenesis: from development to pathologies. FASEB J 31:4665–4681
Lambert V et al (2013) Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice. Nat Protoc 8:2197–2211
Batson J et al (2017) Development of potent, selective SRPK1 inhibitors as potential topical therapeutics for neovascular eye disease. ACS Chem Biol 12:825–832
Acknowledgments
We would like to thank National Eye Research Charity, British Heart Foundation PG/18/31/33759, and Royal Society RGS\R1\191221 for their generous funding.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Green, K.R., Beazley-Long, N., Lynch, A.P., Allen, C.L., Bates, D.O., Benest, A.V. (2022). Quantification of Angiogenesis in Laser Choroidal Neovascularization. In: Benest, A.V. (eds) Angiogenesis. Methods in Molecular Biology, vol 2441. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2059-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2059-5_17
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2058-8
Online ISBN: 978-1-0716-2059-5
eBook Packages: Springer Protocols