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Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2437))

Abstract

Mass spectrometry imaging (MSI) plays an expanding role in the label-free spatial mapping of hundreds of molecules simultaneously. Currently, matrix-assisted laser desorption ionization (MALDI) is among the most widely adopted MSI techniques. However, matrix application can impact the fidelity of spatial distributions, and matrix selection and related spectral interferences in the low mass range can lead to biased molecular coverage. Nanophotonic ionization from silicon nanopost arrays (NAPA) is an emerging matrix-free MSI platform with enhanced sensitivity for several molecular classes, for example, neutral lipids and biooligomers. Here, we describe a protocol with minimal sample preparation for NAPA-MSI of metabolites, lipids, and biooligomers from biological tissues.

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Acknowledgments

This work is supported by the US National Science Foundation, Plant Genome Program under grant no. IoS-1734145.

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Authors and Affiliations

  1. Department of Chemistry, George Washington University, Washington, DC, USA

    Laith Z. Samarah & Akos Vertes

Authors
  1. Laith Z. Samarah
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  2. Akos Vertes
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Corresponding authors

Correspondence to Laith Z. Samarah or Akos Vertes .

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Editors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, USA

    Young-Jin Lee

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Samarah, L.Z., Vertes, A. (2022). Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2030-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2029-8

  • Online ISBN: 978-1-0716-2030-4

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