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Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of Aplysia Californica

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Axonal Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2431))

Abstract

Axonal transport moves proteins, RNAs, and organelles between the soma and synapses to support synaptic function and activity-dependent changes in synaptic strength. This transport is impaired in several neurodegenerative disorders such as Alzheimer’s disease. Thus, it is critical to understand the regulation and underlying mechanisms of the transport process. Aplysia californica provides a powerful experimental system for studying the interplay between synaptic activity and transport because its defined synaptic circuits can be built in-vitro. Advantages include precise pre- and postsynaptic manipulation, and high-resolution imaging of axonal transport. Here, we describe methodologies for the quantitative analysis of axonal transport in Aplysia sensory neurons.

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Acknowledgments

We gratefully acknowledge funding support from NIH (5R01MH094607-05, 5R21MH108929-02), and NSF (Award number 1453799) to carry out this work.

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Authors and Affiliations

  1. Department of Neuroscience, The Scripps Research Institute, Scripps Florida, Jupiter, FL, USA

    Kerriann Badal, Yibo Zhao & Sathyanarayanan V. Puthanveettil

  2. Integrated Biology Program, Florida Atlantic University, Jupiter, FL, USA

    Kerriann Badal

  3. Department of Integrative Biology, Michigan State University, East Lansing, MI, USA

    Kyle E. Miller

Authors
  1. Kerriann Badal
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  2. Yibo Zhao
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  3. Kyle E. Miller
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  4. Sathyanarayanan V. Puthanveettil
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Corresponding authors

Correspondence to Kyle E. Miller or Sathyanarayanan V. Puthanveettil .

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Editors and Affiliations

  1. Department of Basic and Clinical Neurosciences, Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, London, UK

    Alessio Vagnoni

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Badal, K., Zhao, Y., Miller, K.E., Puthanveettil, S.V. (2022). Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of Aplysia Californica. In: Vagnoni, A. (eds) Axonal Transport. Methods in Molecular Biology, vol 2431. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1990-2_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1990-2_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1989-6

  • Online ISBN: 978-1-0716-1990-2

  • eBook Packages: Springer Protocols

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