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In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay

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Stem Cell Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2429))

Abstract

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. In this chapter, we provide a detailed method for the quantification of CSCs in vitro through mammosphere formation.

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References

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Acknowledgments

This work was supported by the NIH/NCI grants 2R01CA155243; 1R01CA200970 awarded to S.A.M, and the NIH T32 grant 5T32CA186892 to N.A.K.

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Authors and Affiliations

  1. Forbes Institute for Cancer Discovery, University of Michigan Medical School, Ann Arbor, MI, USA

    Max S. Wicha

  2. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Nick A. Kuburich, Petra den Hollander, Abhijeet P. Deshmukh, Suhas Vasaikar, Robiya Joseph & Sendurai A. Mani

Authors
  1. Nick A. Kuburich
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  2. Petra den Hollander
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  3. Abhijeet P. Deshmukh
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  4. Suhas Vasaikar
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  5. Robiya Joseph
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  6. Max S. Wicha
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  7. Sendurai A. Mani
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Corresponding author

Correspondence to Sendurai A. Mani .

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Editors and Affiliations

  1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Nagarajan Kannan

  2. Hull York Medical School, York, UK

    Philip Beer

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Kuburich, N.A. et al. (2022). In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_35

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  • DOI: https://doi.org/10.1007/978-1-0716-1979-7_35

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1978-0

  • Online ISBN: 978-1-0716-1979-7

  • eBook Packages: Springer Protocols

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