Abstract
The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.
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Acknowledgments
M.G. and J.L. are funded by the Gatsby Charitable Foundation (grant number GAT3395/GLC). A.H. is funded by the BBSRC (grant number BB/P016774/1).
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Greenwood, M., Hall, A.J.W., Locke, J.C.W. (2022). High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock. In: Staiger, D., Davis, S., Davis, A.M. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 2398. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1912-4_4
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DOI: https://doi.org/10.1007/978-1-0716-1912-4_4
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