Abstract
The opsonophagocytic killing assay (OPKA) is designed to measure the functionality of strain-specific antibodies and, therefore, assess protective immunity or the immunogenicity of Group A Streptococcus (GAS) (type A Streptococcus pyogenes) vaccines. Opsonization of GAS for phagocytosis is an important mechanism by which antibodies protect against disease in vivo. The Opsonophagocytic Index or Opsonic Index (OI) is the estimated dilution of antisera that kills 50% of the target bacteria. Here, we describe the protocol of the standardized GAS OPKA developed by Jones et al., 2018.
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References
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Wagstaffe, H., Jones, S., Johnson, M., Goldblatt, D. (2022). Opsonophagocytic Killing Assay to Measure Anti–Group A Streptococcus Antibody Functionality in Human Serum. In: Bidmos, F., Bossé, J., Langford, P. (eds) Bacterial Vaccines. Methods in Molecular Biology, vol 2414. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1900-1_20
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DOI: https://doi.org/10.1007/978-1-0716-1900-1_20
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1899-8
Online ISBN: 978-1-0716-1900-1
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