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Biomedical Engineering Technologies pp 751–771Cite as

In Vivo Quantitative Whole-Body Perfusion Imaging Using Radiolabeled Copper(II) Bis(Thiosemicarbazone) Complexes and Positron Emission Tomography (PET)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2393))

Abstract

Traditional quantitative perfusion imaging methods require complex data acquisition and analysis strategies; typically require ancillary arterial blood sampling for measurement of input functions; are limited to single organ or tissue regions in an imaging session; and because of their complexity, are not well suited for routine clinical implementation in a standardized fashion that can be readily repeated across diverse clinical sites. The whole-body perfusion method described in this chapter has the advantages of on-demand radiotracer production; simple tissue pharmacokinetics enabling standardized estimation of perfusion; short-lived radionuclides, facilitating repeat or combination imaging procedures; and scalability to support widespread clinical implementation. This method leverages the unique physiological characteristics of radiolabeled copper(II) bis(thiosemicarbazone) complexes and the detection sensitivity of positron emission tomography (PET) to produce quantitatively accurate whole-body perfusion images. This chapter describes the synthesis of ethylglyoxal bis(thosemicarbazonato)copper(II) labeled with copper-62 ([62Cu]Cu-ETS), its unique physiological characteristics, a simple tracer kinetic model for estimation of perfusion using image-derived input functions, and validation of the method against a reference standard perfusion tracer. A detailed description of the methods is provided to facilitate implementation of the perfusion imaging method in PET imaging facilities.

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Acknowledgements

This work was supported by grants from the National Cancer Institute of the National Institutes of Health (CA202695 and CA140299), a National Institutes of Health Short-Term Training Program in Biomedical Sciences Grant (T35 HL110854), and a Radiological Society of North America (RSNA) Research & Education Foundation Research Medical Student Grant Award (Monica Cheng).

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Authors and Affiliations

  1. Department of Radiology and Imaging Sciences, Indiana University School of Medicine, Indianapolis, IN, USA

    Mark A. Green, Carla J. Mathias, Nathaniel J. Smith, Monica Cheng & Gary D. Hutchins

Authors
  1. Mark A. Green
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  2. Carla J. Mathias
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  3. Nathaniel J. Smith
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  4. Monica Cheng
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  5. Gary D. Hutchins
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Corresponding author

Correspondence to Gary D. Hutchins .

Editor information

Editors and Affiliations

  1. Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, MD, USA

    Miguel R. Ossandon

  2. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Houston Baker

  3. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Avraham Rasooly

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Green, M.A., Mathias, C.J., Smith, N.J., Cheng, M., Hutchins, G.D. (2022). In Vivo Quantitative Whole-Body Perfusion Imaging Using Radiolabeled Copper(II) Bis(Thiosemicarbazone) Complexes and Positron Emission Tomography (PET). In: Ossandon, M.R., Baker, H., Rasooly, A. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2393. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1803-5_40

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  • DOI: https://doi.org/10.1007/978-1-0716-1803-5_40

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1802-8

  • Online ISBN: 978-1-0716-1803-5

  • eBook Packages: Springer Protocols

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