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Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing

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Plant Circular RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2362))

Abstract

Lariat RNAs are well-known by-products of pre-mRNA splicing in eukaryotes, which are produced by the excised introns when the 5′ splice site (5′ ss) joins with the branchpoint (BP) during splicing. In general, most of lariat RNAs are usually linearized by RNA debranching enzyme 1 (DBR1), followed by degradation for intron turnover. However, with the high-throughput RNA sequencing technology and bioinformatics methods, increasing evidences have shown that many lariat RNAs can stably accumulate under physiological conditions in both animals and plants. Here, we describe a large-scale analysis to systematically identify the lariat RNAs (i.e., intronic circular RNAs) in Arabidopsis by utilizing the RNA-sequencing data.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China

    Taiyun Wang, Xiaotuo Zhang & Binglian Zheng

Authors
  1. Taiyun Wang
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  2. Xiaotuo Zhang
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  3. Binglian Zheng
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Corresponding author

Correspondence to Binglian Zheng .

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Editors and Affiliations

  1. Oncativo, Argentina

    Luis María Vaschetto

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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Wang, T., Zhang, X., Zheng, B. (2021). Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing. In: Vaschetto, L.M. (eds) Plant Circular RNAs. Methods in Molecular Biology, vol 2362. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1645-1_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1645-1_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1644-4

  • Online ISBN: 978-1-0716-1645-1

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