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Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining

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Cardiovascular Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2319))

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Abstract

The blood vascular system is a tree-like hierarchical branching structure and needs to function even before fully established. Abnormal formation of blood vessels results in embryonic lethality and also contributes to the pathogenesis of a number of human diseases, including cancer metastasis. To understand the molecular events associated with blood vessel formation, we established a fluorescence staining-based protocol on mouse embryonic skin. We harvested mouse embryonic skin and performed whole-mount staining. The reconstructed three-dimensional vascular structure provided detailed information on angiogenesis.

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References

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Acknowledgments

This work was supported by American Heart Association Transformational Project Award (19TPA34900011) to Xu Peng.

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Author notes

  1. Jian Wang and Yuwei Dong contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA

    Jian Wang, Yuwei Dong, Yu Xi & Xu Peng

Authors
  1. Jian Wang
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  2. Yuwei Dong
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  3. Yu Xi
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  4. Xu Peng
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Corresponding author

Correspondence to Xu Peng .

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Editors and Affiliations

  1. Med. Physiology, Health Science Center, Texas A&M University, Bryan, TX, USA

    Xu Peng

  2. Med. Physiology, Health Science Center, Texas A&M University, Bryan, TX, USA

    Warren E. Zimmer

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Wang, J., Dong, Y., Xi, Y., Peng, X. (2021). Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining. In: Peng, X., Zimmer, W.E. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 2319. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1480-8_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1480-8_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1479-2

  • Online ISBN: 978-1-0716-1480-8

  • eBook Packages: Springer Protocols

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