Abstract
Laser capture microdissection (LCM) enables researchers to selectively evaluate gene expression profiling of a specific cell type within a tissue. Vascular endothelial cells (EC) line the inside of vessel lumen and play an essential role in new blood vessel formation. It remains a challenge to determine vascular ECs-specific genes expression in vivo. Here, we described a method to dissect vascular ECs from the frozen heart tissue by LCM. The total RNA or proteins are then extracted from the ECs for further analysis.
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References
Espina V, Wulfkuhle JD, Calvert VS, VanMeter A, Zhou W, Coukos G et al (2006) Laser-capture microdissection. Nat Protoc 1(2):586–603
Heinmoller E, Schlake G, Renke B, Liu Q, Hill KA, Sommer SS et al (2002) Microdissection and molecular analysis of single cells or small cell clusters in pathology and diagnosis--significance and challenges. Anal Cell Pathol 24(4–5):125–134
Fend F, Raffeld M (2000) Laser capture microdissection in pathology. J Clin Pathol 53(9):666–672
Bertos NR, Park M (2016) Laser capture microdissection as a tool to study tumor stroma. Methods Mol Biol 1458:13–25
Ruan W, Sassoon A, An F, Simko JP, Liu B (2006) Identification of clinically significant tumor antigens by selecting phage antibody library on tumor cells in situ using laser capture microdissection. Mol Cell Proteomics 5(12):2364–2373
Pellegrini D, Del Grosso A, Angella L, Giordano N, Dilillo M, Tonazzini I et al (2019) Quantitative microproteomics based characterization of the central and peripheral nervous system of a mouse model of Krabbe disease. Mol Cell Proteomics 18(6):1227–1241
Goodall EF, Leach V, Wang C, Cooper-Knock J, Heath PR, Baker D et al (2019) Age-associated mRNA and miRNA expression changes in the blood-brain barrier. Int J Mol Sci 20(12):3097
Funel N, Giovannetti E, Pollina LE, del Chiaro M, Mosca F, Boggi U et al (2011) Critical role of laser microdissection for genetic, epigenetic and proteomic analyses in pancreatic cancer. Expert Rev Mol Diagn 11(7):695–701
Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci Int 137(1):28–36
Costa S, Correia-de-Sa P, Porto MJ, Caine L (2017) The use of laser microdissection in forensic sexual assault casework: pros and cons compared to standard methods. J Forensic Sci 62(4):998–1006
Miller JA, Ding SL, Sunkin SM, Smith KA, Ng L, Szafer A et al (2014) Transcriptional landscape of the prenatal human brain. Nature 508(7495):199–206
Tayade C, Black GP, Fang Y, Croy BA (2006) Differential gene expression in endometrium, endometrial lymphocytes, and trophoblasts during successful and abortive embryo implantation. J Immunol 176(1):148–156
Vandewoestyne M, Goossens K, Burvenich C, Van Soom A, Peelman L, Deforce D (2013) Laser capture microdissection: should an ultraviolet or infrared laser be used? Anal Biochem 439(2):88–98
Datta S, Malhotra L, Dickerson R, Chaffee S, Sen CK, Roy S (2015) Laser capture microdissection: big data from small samples. Histol Histopathol 30(11):1255–1269
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Zhou, T., Wang, J. (2021). Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues. In: Peng, X., Zimmer, W.E. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 2319. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1480-8_12
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DOI: https://doi.org/10.1007/978-1-0716-1480-8_12
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