Abstract
Here we present a protocol of interest to those who want to look at the functional consequences of decreasing expression of essential genes constitutively as well as being able to study the regulatory pathways of that essential gene by also deleting one or two additional genes. This allows epistasis relationships to be determined.
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Acknowledgments
We thank Manning Huang for design of the experimental flowchart. This work was supported by NIH grants R21 AI100270 (A.P.M.) and R01 AI067703 (A.P.M.). Protocol first published in Woolford et al. [3].
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Woolford, C.A., Mitchell, A.P. (2021). Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans . In: Bignell, E. (eds) Host-Fungal Interactions. Methods in Molecular Biology, vol 2260. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1182-1_1
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DOI: https://doi.org/10.1007/978-1-0716-1182-1_1
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