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Mapping DNA Methylation in Mammals: The State of the Art

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DNA Modifications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2198))

Abstract

A complete understanding of the dynamics and function of cytosine modifications in mammalian biology is lacking. Central to achieving this understanding is the availability of techniques that permit sensitive and specific genome-wide mapping of DNA modifications in mammalian DNA. The last decade has seen the development of a vast arsenal of novel profiling approaches enabling epigeneticists to tackle research questions that were previously out of reach. Here, we review the techniques currently available for profiling DNA modifications in mammals, discuss their strengths and weaknesses, and speculate on the future direction of DNA modification profiling technologies.

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Authors and Affiliations

  1. Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden

    Antonio Lentini

  2. Department of Biomedical and Clinical Sciences (BKV), Crown Princess Victoria Children’s Hospital, Linköping University, Linköping, Sweden

    Colm E. Nestor

Authors
  1. Antonio Lentini
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  2. Colm E. Nestor
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Correspondence to Colm E. Nestor .

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Editors and Affiliations

  1. Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham, UK

    Alexey Ruzov

  2. School of Life Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham, UK

    Martin Gering

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© 2021 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Lentini, A., Nestor, C.E. (2021). Mapping DNA Methylation in Mammals: The State of the Art. In: Ruzov, A., Gering, M. (eds) DNA Modifications. Methods in Molecular Biology, vol 2198. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0876-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0876-0_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0875-3

  • Online ISBN: 978-1-0716-0876-0

  • eBook Packages: Springer Protocols

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