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Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling

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Book cover Cancer Cell Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2174))

Abstract

With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. Here, we provide a collection of detailed experimental protocols for lncRNA studies, including lncRNA immunoprecipitation, lncRNA pull-down, lncRNA northern blot analysis, lncRNA in situ hybridization, and lncRNA knockdown. We hope that the information included in this chapter can speed up research on lncRNAs biology and eventually lead to the development of clinical applications with lncRNA as novel prognostic markers and therapeutic targets.

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Acknowledgments

This work was supported, in whole or in part, the US National Institutes of Health (R01CA225929 to LZ, R01CA142776 to LZ, R01CA190415 to LZ, P50CA083638 to LZ, P50CA174523 to LZ), the Breast Cancer Alliance (LZ), the Marsha Rivkin Center for Ovarian Cancer Research (LZ), the Harry Fields Professorship (LZ), the Kaleidoscope of Hope Ovarian Cancer Foundation (LZ), the Ovarian Cancer Research Fund Alliance (XH), and the Foundation for Women’s Cancer (XH and Z).

Author information

Authors and Affiliations

  1. Center for Reproduction and Women’s Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan, Mu Xu, Chunsheng Li, Youyou Zhang, Lin Zhang & Xiaowen Hu

  2. Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Lin Zhang & Xiaowen Hu

Authors
  1. Yi Feng
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  2. Junjie Jiang
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  3. Zhongyi Hu
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  5. Tianli Zhang
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  6. Yutian Pan
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  7. Mu Xu
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  8. Chunsheng Li
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  9. Youyou Zhang
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  10. Lin Zhang
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  11. Xiaowen Hu
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Corresponding author

Correspondence to Xiaowen Hu .

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Editors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico

    Martha Robles-Flores

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Feng, Y. et al. (2021). Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling. In: Robles-Flores, M. (eds) Cancer Cell Signaling. Methods in Molecular Biology, vol 2174. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0759-6_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0759-6_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0758-9

  • Online ISBN: 978-1-0716-0759-6

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