Abstract
Mitochondrial fission is mainly regulated by a number of dynamin superfamily proteins or DSPs, of which dynamin-like protein 1 (Drp1) is responsible for the scission process during mitochondrial fission. Here we describe several methods, including monitoring mitochondrial distribution, phosphorylation, and tetramer level of Drp1, to examine the activity of Drp1 in mitochondrial fission in vivo.
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Acknowledgment
This work was supported by NIH R01 NS088192 to X.Q.
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Hu, D., Qi, X. (2020). Measuring Drp1 Activity in Mitochondrial Fission In Vivo. In: Ramachandran, R. (eds) Dynamin Superfamily GTPases. Methods in Molecular Biology, vol 2159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0676-6_14
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DOI: https://doi.org/10.1007/978-1-0716-0676-6_14
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0676-6
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