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Culturing and Manipulating Mouse Embryonic Stem Cells

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Stem Cells and Tissue Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2155))

Abstract

Mouse embryonic stem cells (mESC) have the ability to self-renew due to their rapid proliferation and high telomerase activity while maintaining their pluripotency. Depending on the environment, mESC can differentiate into a broad range of cell types. These characteristics have established mESC as a tool for modeling human disease, genetic engineering, lineage specificity, stem cell-based therapies, and tissue regeneration. Here we describe a protocol for mESC expansion and differentiation.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA

    Chrissa Kioussi

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  1. Chrissa Kioussi
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Correspondence to Chrissa Kioussi .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA

    Chrissa Kioussi

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Kioussi, C. (2020). Culturing and Manipulating Mouse Embryonic Stem Cells. In: Kioussi, C. (eds) Stem Cells and Tissue Repair . Methods in Molecular Biology, vol 2155. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0655-1_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0655-1_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0654-4

  • Online ISBN: 978-1-0716-0655-1

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