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Biolistic Approach for Transient Gene Expression Studies in Plants

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Biolistic DNA Delivery in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2124))

Abstract

Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research. Here, we review the technical aspects and the major applications of the biolistic method for studies involving transient gene expression in plant cells. These studies cover multiple areas of plant research, including gene expression, protein subcellular localization and cell-to-cell movement, plant virology, silencing, and the more recently developed targeted genome editing via transient expression of customized endonucleases.

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Acknowledgments

The work in the VC laboratory is supported by grants from USDA/NIFA, NIH, NSF, and BARD to V.C.

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Authors and Affiliations

  1. Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY, USA

    Benoît Lacroix & Vitaly Citovsky

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  1. Benoît Lacroix
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  2. Vitaly Citovsky
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Correspondence to Benoît Lacroix .

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Editors and Affiliations

  1. Department of Plant and Environmental Sciences, School of Health Research, Pee Dee Research and Education Center, Clemson University, Florence, SC, USA

    Sachin Rustgi

  2. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA

    Hong Luo

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Lacroix, B., Citovsky, V. (2020). Biolistic Approach for Transient Gene Expression Studies in Plants. In: Rustgi, S., Luo, H. (eds) Biolistic DNA Delivery in Plants. Methods in Molecular Biology, vol 2124. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0356-7_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0356-7_6

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