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Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications

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Book cover Biolistic DNA Delivery in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2124))

Abstract

The present and subsequent chapters in this volume are dedicated to the life and research of Professor Diter von Wettstein who contributed immensely to the development of science and education. His contributions spanned various fields of science such as genetics, physiology, ultrastructural analysis, molecular biology, genomics, and biotechnology including genome editing. He performed and promoted pioneering research in the fields of epigenetics, directed evolution of enzymes, synthetic biology (promoter and gene optimizations), and genomics (genome sequencing of baker’s yeast). Glimpses of his time at the Carlsberg Laboratory and Washington State University, with examples from the research performed at these institutions, are included in this chapter. His life is an inspiration to the next generation of biologists. Despite difficult situations, his persistent efforts and keen desire to learn enabled him to overcome obstacles. He always tried to attain the best, excelling in translating fundamental knowledge into applications.

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References

  1. von Wettstein D (2007) From analysis of mutants to genetic engineering. Annu Rev Plant Biol 58:1–19

    Article  Google Scholar 

  2. Wen S, Wen N, Pang J, Langen G, Brew-Appiah RAT, Mejias JH, Osorio C, Yang MM, Gemini R, Moehs CP, Zemetra RS, Kogel KH, Liu B, Wang X, von Wettstein D, Rustgi S (2012) Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health. Proc Natl Acad Sci U S A 109:20543–20548

    Article  CAS  Google Scholar 

  3. Rustgi S, Wen N, Osorio C, Brew-Appiah RAT, Wen S, Gemini R, Mejias JH, Ankrah N, Moehs CP, von Wettstein D (2014) Natural dietary therapies for the ‘gluten syndrome’. The Royal Danish Academy of Sciences and Letters, Copenhagen

    Google Scholar 

  4. Osorio C, Wen N, Mejias JH, Liu B, Reinbothe S, von Wettstein D, Rustgi S (2019) Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac disease. Funct Integr Genomics 19:123–136

    Article  CAS  Google Scholar 

  5. Horvath H, Huang J, Wong OT, von Wettstein D (2002) Experiences with genetic transformation of barley and characteristics of transgenic plants. In: Slafer GA, Molina-Cano JL, Savin R, Araus JL, Romagosa I (eds) . Food Products Press, Barley science resent advances from molecular biology to agronomy of yield and quality, pp 143–176

    Google Scholar 

  6. Borriss R, Olsen O, Thomsen KK, von Wettstein D (1989) Hybrid bacillus endo-(1–3,1–4)-β-glucanases: construction of recombinant genes and molecular properties of the gene products. Carlsb Res Commun 54:41–54

    Article  CAS  Google Scholar 

  7. The Royal Swedish Academy of Sciences (2018) Scientific background on the Nobel prize in chemistry 2018 – directed evolution of enzymes and binding proteins. https://old.nobelprize.org/che-sci.pdf?_ga=2.228216788.850160180.1538560699-1556534510.1538560699

    Google Scholar 

  8. von Wettstein D (2006) Fascination with chloroplasts and chromosome pairing. Progress Bot 67:3–28

    Article  Google Scholar 

  9. Hoober JK (2017) Diter von Wettstein (Dietrich Holger Wettstein Ritter von Westersheim): September 20, 1929–April 13, 2017. Photosynth Res 134:107–110

    Article  CAS  Google Scholar 

  10. Zickler D (2020) Diter von Wettstein and the meiotic program of pairing and recombination. In: Rustgi S, Luo H (eds) Biolistic DNA delivery in plants. Springer, New York, NY (chapter 2, this volume)

    Google Scholar 

  11. Kogel KH, Voll LM, Schäfer P, Jansen C, Wu Y, Langen G, Imani J, Hofmann J, Schmiedl A, Sonnewald S, von Wettstein D, Cook RJ, Sonnewald U (2010) Transcriptome and metabolome profiling of field-grown transgenic barley lack induced differences but show cultivar-specific variances. Proc Natl Acad Sci U S A 107:6198–6203

    Article  CAS  Google Scholar 

  12. Brew-Appiah RAT, Ankrah N, Liu W, Konzak CF, von Wettstein D, Rustgi S (2013) Generation of doubled haploid transgenic wheat lines by microspore transformation. PLoS One 8:e80155

    Article  Google Scholar 

  13. Rustgi S, von Wettstein D (2015) Breeding barley ornamented with the novel agronomical attributes. Med Aromat Plants 4:2

    Google Scholar 

  14. Rustgi S, Kashyap S, Gandhi N, von Wettstein D, Ankrah N, Gemini R, Reisenauer P (2018) Novel wheat genotypes designed to meet the future needs for safe and surplus food. Ann Wheat Newslet 64:56–61

    Google Scholar 

  15. Rustgi S, Kashyap S, Ankrah N, von Wettstein D (2020) Use of microspore-derived calli as explants for biolistic transformation of common wheat. In: Rustgi S, Luo H (eds) Biolistic DNA delivery in plants. Springer, New York, NY (chapter 14, this volume)

    Google Scholar 

  16. Lee H, Rustgi S, Kumar N, Burke I, Yenish JP, Gill KS, von Wettstein D, Ullrich SE (2011) Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides. Proc Natl Acad Sci U S A 108:8909–8913

    Article  CAS  Google Scholar 

  17. Rustgi S, Matanguihan J, Mejias JH, Gemini R, Brew-Appiah RAT, Wen N, Osorio C, Ankrah N, Murphy KM, von Wettstein D (2014) Assessment of genetic diversity among barley cultivars and breeding lines adapted to the US Pacific Northwest, and its implications in breeding barley for imidazolinone-resistance. PLoS One 9:e100998

    Article  Google Scholar 

  18. von Wettstein D, Cochran JS, Ullrich SE, Kannangara CG, Jitkov VA, Burns JW, Reisenauer PE, Chen X, Jones BL (2004) Registration of ‘Radiant’ barley. Crop Sci 44:1859–1860

    Article  Google Scholar 

  19. Rustgi S, Brouwer B, von Wettstein D, Reisenauer PE, Lyon S, Ankrah N, Jones S, Guy SO, Chen X (2020) Registration of ‘Fritz’, a two-row, spring barley. J Plant Registr. (under review)

    Google Scholar 

  20. Jensen LG, Olsen O, Kops O, Wolf N, Thomsen KK, von Wettstein D (1996) Transgenic barley expressing a protein-engineered, thermostable (1,3-1,4)-beta-glucanase during germination. Proc Natl Acad Sci U S A 93:3487–3491

    Article  CAS  Google Scholar 

  21. Jensen LG, Politz O, Olsen O, Thomsen KK, von Wettstein D (1998) Inheritance of a codon-optimized transgene expressing heat stable (1,3-1,4)-β-glucanase in scutellum and aleurone of germinating barley. Hereditas 129:215–225

    Article  CAS  Google Scholar 

  22. Horvath H, Huang J, Wong O, Kohl E, Okita T, Kannangara CG, von Wettstein D (2000) The production of recombinant proteins in transgenic barley grains. Proc Natl Acad Sci U S A 97:1914–1919

    Article  CAS  Google Scholar 

  23. von Wettstein D, Mikhaylenko G, Froseth JA, Kannangara CG (2000) Improved barley broiler feed with transgenic malt containing heat-stable (1,3-1,4)-beta-glucanase. Proc Natl Acad Sci U S A 97:13512–13517

    Article  Google Scholar 

  24. von Wettstein D, Warner J, Kannangara CG (2003) Supplements of transgenic malt or grain containing (1,3-1,4)-β-glucanase to barley based broiler diets lift their nutritive value to that of corn. Br J Poult Sci 44:438–449

    Article  Google Scholar 

  25. Wu Y, von Wettstein D, Kannangara CG, Nirmala J, Cook RJ (2006) Growth inhibition of the cereal root pathogens Rhizoctonia solani AG8, R. oryzae and Gaeumannomyces graminis var. tritici by a recombinant endochitinase from Trichoderma harzianum. Biocontrol Sci Tech 16:631–646

    Article  Google Scholar 

  26. Osorio C (2004) Development of transgenic barley expressing human type I collagen. M.Sc., thesis, Department of Crop and Soil Sciences, Washington State University, Pullman WA

    Google Scholar 

  27. Stahl R, Horvath H, Van Fleet J, Voetz M, von Wettstein D, Wolf N (2002) T-DNA integration into the barley genome from single and double cassette vectors. Proc Natl Acad Sci U S A 99:2146–2151

    Article  CAS  Google Scholar 

  28. Horvath H, Rostoks N, Brueggeman R, Steffenson B, von Wettstein D, Kleinhofs A (2003) Genetically engineered stem rust resistance in barley using the Rpg1 gene. Proc Natl Acad Sci U S A 100:364–369

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Penny von Wettstein-Knowles for her support, corrections, and providing pictures used in necrology and the financial support from the State of South Carolina grant S009, the Life Sciences Discovery Fund Grant 3143956-01, and the Clemson Faculty Succeeds Grant 1502211.

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Correspondence to Sachin Rustgi .

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Rustgi, S., Skadhauge, B. (2020). Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications. In: Rustgi, S., Luo, H. (eds) Biolistic DNA Delivery in Plants. Methods in Molecular Biology, vol 2124. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0356-7_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0356-7_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0355-0

  • Online ISBN: 978-1-0716-0356-7

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