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Establishment of a Standardized Vaccine Protocol for the Analysis of Protective Immune Responses During Experimental Trypanosome Infections in Mice

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Trypanosomatids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2116))

Abstract

To date, trypanosomosis control in humans and animals is achieved by a combination of parasitological screening and treatment. While this approach has successfully brought down the number of reported T. b. gambiense Human African Trypanosomosis (HAT) cases, the method does not offer a sustainable solution for animal trypanosomosis (AT). The main reasons for this are (i) the worldwide distribution of AT, (ii) the wide range of insect vectors involved in transmission of AT, and (iii) the existence of a wildlife parasite reservoir that can serve as a source for livestock reinfection. Hence, in order to control livestock trypanosomosis the only viable long-term solution is an effective antitrypanosome vaccination strategy. Over the last decades, multiple vaccine approaches have been proposed. Despite repeated reports of promising experimental approaches, none of those made it to a field applicable vaccine format. This failure can in part be attributed to flaws in the experimental design that favor a positive laboratory result. This chapter provides a vaccine protocol that should allow for a proper outcome prediction in experimental anti-AT vaccine approaches.

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Acknowledgments

Trypanosome vaccine research at the Magez laboratory is being supported by two grants of the FWO (Fonds voor Wetenschappelijk Onderzoek—Vlaanderen) # G015016 N and #G013518 N.

Author information

Authors and Affiliations

  1. Biomedical Research Centre, Department of Environmental Technology, Food Technology and Molecular Biotechnology, Ghent University, Incheon, South Korea

    Magdalena Radwanska, Hang Thi Thu Nguyen, Sangphil Moon, Emmanuel Obishakin & Stefan Magez

  2. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium

    Magdalena Radwanska & Sangphil Moon

  3. Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium

    Hang Thi Thu Nguyen & Stefan Magez

  4. Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium

    Hang Thi Thu Nguyen, Sangphil Moon & Stefan Magez

  5. Biotechnology Division, National Veterinary Research Institute, Vom, Plateau, Nigeria

    Emmanuel Obishakin

Authors
  1. Magdalena Radwanska
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  2. Hang Thi Thu Nguyen
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  3. Sangphil Moon
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  4. Emmanuel Obishakin
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  5. Stefan Magez
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Corresponding author

Correspondence to Stefan Magez .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Paul A. M. Michels

  2. School of Applied Sciences, University of Huddersfield, Huddersfield, UK

    Michael L. Ginger

  3. Faculty of Biology, Technion–Israel Institute of Technology, Haifa, Israel

    Dan Zilberstein

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Radwanska, M., Nguyen, H.T.T., Moon, S., Obishakin, E., Magez, S. (2020). Establishment of a Standardized Vaccine Protocol for the Analysis of Protective Immune Responses During Experimental Trypanosome Infections in Mice. In: Michels, P., Ginger, M., Zilberstein, D. (eds) Trypanosomatids. Methods in Molecular Biology, vol 2116. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0294-2_42

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  • DOI: https://doi.org/10.1007/978-1-0716-0294-2_42

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0293-5

  • Online ISBN: 978-1-0716-0294-2

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