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Quantitative Phosphoproteomic Analysis of Legume Using TiO2-Based Enrichment Coupled with Isobaric Labeling

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Legume Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2107))

Abstract

Phosphorylation of proteins is the most dynamic protein modification, and its analysis aids in determining the functional and regulatory principles of important cellular pathways. The legumes constitute the third largest family of higher plants, Fabaceae, comprising about 20,000 species and are second to cereals in agricultural importance on the basis of global production. Therefore, an understanding of the developmental and adaptive processes of legumes demands identification of their regulatory components. The most crucial signature of the legume family is the symbiotic nitrogen fixation, which makes this fascinating and interesting to investigate phosphorylation events. The research on protein phosphorylation in legumes has been focused primarily on two model species, Medicago truncatula and Lotus japonicus. The development of reciprocal research in other species, particularly the crops, is lagging behind which has limited its beneficial uses in agricultural productivity. In this chapter, we outline the titanium dioxide-based enrichment of phosphopeptides for nuclear proteome analysis of a grain legume, chickpea.

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Acknowledgments

This work was supported by grants from the Department of Science and Technology (DST)-SERB (EMR/2015/001870), India. The authors thank Department of Biotechnology (DBT) and Council of Scientific and Industrial Research (CSIR), India for providing research fellowship to PB, NVL, and SK, respectively.

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Authors and Affiliations

  1. National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, New Delhi, India

    Pragya Barua, Nilesh Vikram Lande, Sunil Kumar, Subhra Chakraborty & Niranjan Chakraborty

Authors
  1. Pragya Barua
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  2. Nilesh Vikram Lande
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  3. Sunil Kumar
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  4. Subhra Chakraborty
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  5. Niranjan Chakraborty
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Corresponding author

Correspondence to Niranjan Chakraborty .

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Editors and Affiliations

  1. School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India

    Mukesh Jain

  2. Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India

    Rohini Garg

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Barua, P., Lande, N.V., Kumar, S., Chakraborty, S., Chakraborty, N. (2020). Quantitative Phosphoproteomic Analysis of Legume Using TiO2-Based Enrichment Coupled with Isobaric Labeling. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_22

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  • DOI: https://doi.org/10.1007/978-1-0716-0235-5_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0234-8

  • Online ISBN: 978-1-0716-0235-5

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