Abstract
Lentiviral vectors enable gene transfer into target cells, but manufacturing is complex, scale-limited, and costly. Here, we describe the use of microfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.
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Acknowledgments
This work was supported by NIH (R01-HL129141 to W.A.L.) and a research partnership between Children’s Healthcare of Atlanta and the Georgia Institute of Technology. This work was performed in part at the Georgia Tech Institute for Electronics and Nanotechnology, a member of the National Nanotechnology Coordinated Infrastructure, which is supported by the National Science Foundation (grant ECCS-1542174).
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Tran, R., Lam, W.A. (2020). Microfluidic Approach for Highly Efficient Viral Transduction. In: Katz, S., Rabinovich, P. (eds) Cell Reprogramming for Immunotherapy. Methods in Molecular Biology, vol 2097. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0203-4_3
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DOI: https://doi.org/10.1007/978-1-0716-0203-4_3
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