Preservation of clonal genotypes of most fruit crops requires vegetative propagation, so cryopreservation is an ideal method for long-term germplasm storage (Engelmann 2004). Some of the first and most successful experiments with the cryopreservation of apical meristems began with a temperate berry crop, strawberry (Kartha et al. 1980; Sakai et al. 1978). These initial studies showed that slow cooling of <1 mm shoot tips in cryoprotectant solutions was a feasible option for long-term storage of clonally propagated plants. During the last two decades, cryopreservation methods were developed for many species of fruit crops (Reed 2001).
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Reed, B.M. (2008). Cryopreservation of Temperate Berry Crops. In: Reed, B.M. (eds) Plant Cryopreservation: A Practical Guide. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72276-4_13
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